Laboratory of Environmental Physiology, Department of Life Science, School of Science and Engineering, Kindai University, Osaka 577-8502, Japan.
J Reprod Dev. 2020 Apr 10;66(2):143-148. doi: 10.1262/jrd.2019-144. Epub 2019 Dec 29.
High-fat diet (HFD) is associated with the regulation of reproductive functions. This study aimed to investigate the effects of short-term HFD on the mRNA expression levels of follicle-stimulating hormone β subunit (FSHβ), luteinizing hormone β subunit (LHβ), gonadotropin-releasing hormone receptor, and long-chain fatty acid receptor, GPR120, in the matured male mouse pituitary gland. Adult male mice were fed either control chow or HFD for 1, 2, 5, 10, 30 and 150 days. Fshb and Gpr120 mRNA expression levels in the pituitary glands were significantly increased during 2 to 30 days of HFD feeding. Gnrh-r mRNA in the 30 days HFD fed group and body weight in the 30 and 150 days HFD fed groups were higher than control. However, there were no significant differences in plasma non-esterified fatty acids or glucose levels during the 150 days of HFD feeding. These results suggest that male mice feeding a short-term HFD induces FSHβ synthesis and GPR120 expression in their pituitary gonadotropes.
高脂肪饮食（HFD）与生殖功能的调节有关。本研究旨在探讨短期 HFD 对成熟雄性小鼠垂体中卵泡刺激素β亚基（FSHβ）、黄体生成素β亚基（LHβ）、促性腺激素释放激素受体和长链脂肪酸受体 GPR120 的 mRNA 表达水平的影响。成年雄性小鼠分别喂食对照饲料或 HFD，持续 1、2、5、10、30 和 150 天。在 HFD 喂养的 2 至 30 天期间，垂体中 Fshb 和 Gpr120 的 mRNA 表达水平显著增加。在 30 天 HFD 喂养组中，Gnrh-r mRNA 和体重在 30 天和 150 天 HFD 喂养组中均高于对照组。然而，在 150 天 HFD 喂养期间，血浆非酯化脂肪酸或葡萄糖水平没有显著差异。这些结果表明，雄性小鼠短期 HFD 喂养会诱导其垂体促性腺激素中 FSHβ的合成和 GPR120 的表达。
