Toda Takeshi, Hanesaka Shun, Shishido Kuniaki, Fuji Shin-Ichi, Furuya Hiromitsu
Department of Bioproduction Science, Faculty of Bioresource Science, Akita Prefectural University, 241-438 Kaidobata-Nishi, Shimoshinjo-Nakano, Akita, 010-0195, Japan.
Fukushima Prefecture Office, 2-16 Sugitsuma-cho, Fukushima, 960-8670, Japan.
J Genet Eng Biotechnol. 2020 Jan 6;18(1):1. doi: 10.1186/s43141-019-0015-2.
Primers specific for the hypothetical forma specialis of Fusarium oxysporum were designed to amplify DNA from this pathogenic fungus that infects plants including lilies. The F. oxysporum sequence between the transposal elements han and hop was used for primer design. Three primer pairs designed from this region were confirmed as specific for 24 isolates of F. oxysporum pathogenic to lilies, except for one pathogenic isolates as extraordinary. No amplification was observed from F. oxysporum non-pathogenic to lily, from 12 forma specialis, and 14 fungi and oomycetes concerned with Liliaceae plants. We propose that specific primers designed from this region will be useful to detect isolates of F. oxysporum that are pathogenic to lilies.
针对尖孢镰刀菌假定专化型设计了特异性引物，用于扩增来自这种感染包括百合在内植物的致病真菌的DNA。转座元件han和hop之间的尖孢镰刀菌序列用于引物设计。从该区域设计的三对引物被证实对24株对百合致病的尖孢镰刀菌具有特异性，只有一株致病分离株情况特殊。未从对百合无致病性的尖孢镰刀菌、12个专化型以及14种与百合科植物有关的真菌和卵菌中观察到扩增。我们认为，从该区域设计的特异性引物将有助于检测对百合致病的尖孢镰刀菌分离株。
