Montana State University-Bozeman, Department of Research Centers, Western Triangle Agricultural Research Center, Conrad, MT.
Oregon State University, Hermiston Agricultural Research and Extension CenterHermiston, OR.
J Econ Entomol. 2020 Apr 6;113(2):669-678. doi: 10.1093/jee/toz348.
The pea leaf weevil, Sitona lineatus L., is an important pest of field peas and faba beans in most temperate regions. As no information is currently available on efficacy of biopesticides for S. lineatus control, laboratory bioassays were performed to evaluate the impact of biopesticides (spinosad, Beauveria bassiana strain GHA, pyrethrins, B. bassiana GHA + pyrethrins, and B. bassiana GHA + azadirachtin) against adults of this pest. The concentrations used in this bioassay were 0.1, 0.5, 1.0, and 2.0 times the lowest labeled application rate of each product. Results were further verified in cage experiments by assessing biopesticide effects on adult mortality and feeding damage in pea plants. The impact of biopesticides on mortality of larvae of two beneficial species, Chrysoperla carnea and Adalia bipunctata, was also tested in laboratory conditions. We found spinosad to be the most promising candidate, causing 100% adult mortality at high and medium concentrations. Beauveria bassiana and its combination with pyrethrins caused 60-62% adult mortality, but only at the highest concentration. In contrast, B. bassiana + azadirachtin and pyrethrins treatments caused only minimal adult mortality at all concentrations. In cage experiments, spinosad and B. bassiana + pyrethrins had significant effects on adult mortality and provided foliage protection from adult feeding. Conversely, the fungus treatment alone showed inconsistent performance. Beauveria bassiana and spinosad were generally harmless to C. carnea and A. bipunctata larvae, but B. bassiana + pyrethrins was toxic toward beneficial species. These results could help to improve integrated pest management programs intended to control S. lineatus.
豌豆叶甲， Sitona lineatus L.，是大多数温带地区田间豌豆和蚕豆的重要害虫。由于目前尚无关于生物农药防治 S. lineatus 的效果的信息，因此进行了实验室生物测定，以评估生物农药（多杀菌素、球孢白僵菌 GHA 菌株、除虫菊素、球孢白僵菌 GHA + 除虫菊素和球孢白僵菌 GHA + 印苦素）对该害虫成虫的影响。本生物测定中使用的浓度是每种产品最低标签施用量的 0.1、0.5、1.0 和 2.0 倍。结果在笼养实验中通过评估生物农药对豌豆植株上成虫死亡率和取食损伤的影响得到进一步验证。还在实验室条件下测试了生物农药对两种有益物种，Chrysoperla carnea 和 Adalia bipunctata 的幼虫死亡率的影响。我们发现多杀菌素是最有前途的候选者，在高浓度和中浓度下可导致 100%的成虫死亡率。球孢白僵菌及其与除虫菊素的组合可导致 60-62%的成虫死亡率，但仅在最高浓度下。相比之下，B. bassiana + azadirachtin 和除虫菊素处理在所有浓度下仅导致最低限度的成虫死亡率。在笼养实验中，多杀菌素和 B. bassiana + 除虫菊素对成虫死亡率有显著影响，并为叶片提供了免受成虫取食的保护。相反，单独使用真菌处理表现不一致。球孢白僵菌和多杀菌素对 C. carnea 和 A. bipunctata 幼虫一般无害，但 B. bassiana + 除虫菊素对有益物种有毒。这些结果有助于改善旨在控制 S. lineatus 的综合虫害管理计划。
