Department of Neurology, Shandong Provincial Hospital Affiliated to Shandong University, Jinan City, Shandong Province 250021, PR China.
Department of Emergency, Shandong Provincial Hospital Affiliated to Shandong University, Jinan City, Shandong Province 250021, PR China.
Gene. 2020 Mar 20;731:144324. doi: 10.1016/j.gene.2019.144324. Epub 2020 Jan 2.
BACKGROUND/AIMS: lncRNA NEAT1 is involved in the development of many diseases. However, the function of lncRNA NEAT1 in myocardial infarction is unclear. Therefore, this experimental design based on lncRNA NEAT1 to explore the pathogenesis of myocardial infarction.
RT-qPCR was used to detect the expression of lncRNA NEAT1 and miR-378a-3p in peripheral blood and mouse cardiomyocytes of patients with myocardial infarction. MTT assay, flow cytometry, Caspase-3 kit and transwell assay were used to detect the effects of lncRNA NEAT1 and miR-378a-3p on cardiomyocyte proliferation, apoptosis and migration. Target gene prediction and screening, luciferase reporter assays were used to verify downstream target genes for lncRNA NEAT1 and miR-378a-3p. Western blotting was used to detect the protein expression of Atg12 and related autophagy genes.
lncRNA NEAT1 was highly expressed in peripheral blood and mouse cardiomyocytes of patients with myocardial infarction. Moreover, lncRNA NEAT1 significantly promoted cell proliferation and migration of cardiomyocytes. In addition, lncRNA NEAT1 inhibited miR-378a-3p expression, and miR-378a-3p inhibited Atg12 expression, while lncRNA NEAT1 regulated expression of Atg12 and related autophagic factors via miR-378a-3p. Knockout of microRNA-378-3p reversed the effects of NEAT1 silencing on cell damage.
lncRNA NEAT1 can regulate the proliferation of cardiomyocytes by regulating miR-378-3p/Atg12 axis, thus accelerating the occurrence and development of cardiomyocytes.
背景/目的：lncRNA NEAT1 参与多种疾病的发生发展。然而，lncRNA NEAT1 在心肌梗死中的作用尚不清楚。因此，本实验设计基于 lncRNA NEAT1 来探索心肌梗死的发病机制。
采用 RT-qPCR 检测心肌梗死患者外周血和小鼠心肌细胞中 lncRNA NEAT1 和 miR-378a-3p 的表达。采用 MTT 检测、流式细胞术、Caspase-3 试剂盒和 Transwell 检测 lncRNA NEAT1 和 miR-378a-3p 对心肌细胞增殖、凋亡和迁移的影响。采用靶基因预测和筛选、荧光素酶报告实验验证 lncRNA NEAT1 和 miR-378a-3p 的下游靶基因。采用 Western blot 检测 Atg12 及相关自噬基因的蛋白表达。
lncRNA NEAT1 在心肌梗死患者外周血和小鼠心肌细胞中高表达。此外，lncRNA NEAT1 显著促进心肌细胞的增殖和迁移。此外，lncRNA NEAT1 抑制 miR-378a-3p 的表达，而 miR-378a-3p 抑制 Atg12 的表达，而 lncRNA NEAT1 通过 miR-378a-3p 调节 Atg12 和相关自噬因子的表达。敲除 microRNA-378-3p 逆转了 NEAT1 沉默对细胞损伤的影响。
lncRNA NEAT1 可通过调控 miR-378-3p/Atg12 轴调节心肌细胞的增殖，从而加速心肌细胞的发生发展。
