骨再生和矿化在兔下颌骨模型中通过人骨膜蛋白基因促进牵张成骨。
Bone regeneration and mineralisation was promoted during distraction osteogenesis by human periostin gene in rabbit mandibular model.
机构信息
Department of Stomatology, the First People's Hospital of Chenzhou, South Medical University, No. 102, Luojiajing road, 423000, Chenzhou Hunan Province, China; Institute of Translation Medicine, University of China South, China.
Department of Stomatology, the First People's Hospital of Chenzhou, South Medical University, No. 102, Luojiajing road, 423000, Chenzhou Hunan Province, China.
出版信息
J Stomatol Oral Maxillofac Surg. 2020 Nov;121(5):506-511. doi: 10.1016/j.jormas.2019.12.016. Epub 2020 Jan 2.
OBJECTIVE
To study the effect of periostin gene on bone regeneration and mineralisation during distraction osteogenesis (DO) in a rabbit mandibular model.
SUBJECTS AND METHODS
A total of 54 New Zealand white rabbits underwent mandibular lengthening and were divided into group A and group B (n=27, each). At the end of the distraction, adenoviruses containing human POSTN/enhanced green fluorescent protein (Ad5-POSTN/EGFP) viruses or Ad5-POSTN viruses were injected into the distraction gaps in the group B or A, respectively. On the 4th and 8th week after distraction, eight samples, randomly selected from each group, were subjected to CT scanning and dual energy X-ray absorptiometry detection. All animals from each group were sacrificed for micro-CT, histology, and three-point bend testing eight weeks after DO.
RESULTS
The CT images showed that callus in the distraction gap of group B was significantly better than that of group A. On the 4th and 8th weeks, the bone mineral densities (BMD) of the bone tissue in the distraction gap of the group B were 0.163±0.011g/cm and 0.239±0.024g/cm, respectively, while the bone mineral contents (BMC) was 0.099±0.011g and 0.205±0.018g, respectively, which were significantly higher compared with group A. Histological sections showed that the trabecular bone in the distraction gap of group B was more coarse. On the basis of micro-CT images and micro-architecture parameters show that the microstructure of the distraction gap of the group B had more mature characteristics. The biomechanical strength of the distracted gap in the group B was 234.30±30.48N, which was 1.28 times higher than that in the group A (P=0.003).
CONCLUSION
Local transduction with human periostin gene would promote osteogenesis and mineralisation in a rabbit mandibular DO model. Periostin might be considered for clinical use if only there is a safe and effective administration method.
目的
研究外骨膜蛋白（periostin）基因在兔下颌骨牵张成骨（distraction osteogenesis，DO）过程中对骨再生和矿化的影响。
受试者和方法
54 只新西兰白兔行下颌骨延长术，随机分为 A、B 两组（每组 27 只）。牵张结束时，B 组和 A 组分别向牵张间隙内注射携带人 POSTN/增强型绿色荧光蛋白（enhanced green fluorescent protein，EGFP）的腺病毒（adenoviruses containing human POSTN/EGFP，Ad5-POSTN/EGFP）和 Ad5-POSTN 病毒。在牵张后第 4、8 周，每组各随机抽取 8 个样本进行 CT 扫描和双能 X 线吸收法（dual energy X-ray absorptiometry，DXA）检测。所有动物于 DO 后 8 周处死，进行 micro-CT、组织学和三点弯曲试验。
结果
CT 图像显示 B 组牵张间隙内的骨痂明显优于 A 组。第 4、8 周时，B 组牵张间隙内骨组织的骨密度（bone mineral density，BMD）分别为 0.163±0.011g/cm3 和 0.239±0.024g/cm3，骨矿含量（bone mineral content，BMC）分别为 0.099±0.011g 和 0.205±0.018g，均明显高于 A 组。组织学切片显示 B 组牵张间隙内的小梁骨更粗。基于 micro-CT 图像和微结构参数，B 组牵张间隙的微观结构具有更成熟的特征。B 组牵张间隙的生物力学强度为 234.30±30.48N，是 A 组的 1.28 倍（P=0.003）。
结论
局部转染人外骨膜蛋白基因可促进兔下颌骨 DO 模型的成骨和矿化。如果有安全有效的给药方法，外骨膜蛋白可能会被考虑用于临床。
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