Merz W E
Department of Biochemistry II, University of Heidelberg, F.R.G.
Biochem Biophys Res Commun. 1988 Nov 15;156(3):1271-8. doi: 10.1016/s0006-291x(88)80770-8.
Using three different methods evidence was obtained that native and deglycosylated choriogonadotropin show differences in their conformations which might account for the antagonistic properties of deglycosylated choriogonadotropin: 1. In the deglycosylated hormone additional peptide bonds were susceptible to chymotrypsin. 2. In the far ultraviolet circular dichroism only small differences existed between native and deglycosylated choriogonadotropin. However, in 80% hexafluoropropanol the deglycosylated hormone adopted a higher degree of ordered structure. 3. At 37 degrees C the deglycosylated hormone showed a 13 fold increase of the dissociation rate into subunits at pH 3 in comparison to native choriogonadotropin. The results provide evidence that in chemically deglycosylated choriogonadotropin the subunit interactions are disturbed due to conformational changes.
通过三种不同方法获得的证据表明，天然和去糖基化的绒毛膜促性腺激素在构象上存在差异，这可能解释了去糖基化绒毛膜促性腺激素的拮抗特性：1. 在去糖基化激素中，额外的肽键易受胰凝乳蛋白酶作用。2. 在远紫外圆二色性方面，天然和去糖基化绒毛膜促性腺激素之间仅存在微小差异。然而，在80%的六氟丙醇中，去糖基化激素呈现出更高程度的有序结构。3. 在37℃时，与天然绒毛膜促性腺激素相比，去糖基化激素在pH 3条件下亚基解离速率增加了13倍。结果证明，化学去糖基化的绒毛膜促性腺激素中，由于构象变化，亚基间相互作用受到干扰。
