Laurent C, Hellström S, Fellenius E
Department of Anatomy, University of Umeå, Sweden.
Arch Otolaryngol Head Neck Surg. 1988 Dec;114(12):1435-41. doi: 10.1001/archotol.1988.01860240085029.
Preparations of hyaluronan at various concentrations and molecular weights were topically applied to experimental tympanic membrane (TM), perforations in the rat and their ability to improve the healing pattern was elucidated. The perforation occupied the upper posterior quadrant of the TM. Alterations in healing rate and quality of the healed TM were examined by otomicroscopy and in histologic sections. Hyaluronan enhanced the healing rate and resulted in less opacity and structural alteration of the scar area. Closure time for the TM perforation was correlated to the concentration of hyaluronan but not to its molecular weight or viscosity. Improved scar quality was obtained in the presence of hyaluronan irrespective of its rheologic properties. Hyaluronan improved the restoration of the fibrous connective tissue layer. Treatment of TM perforation in man with hyaluronan at high concentrations should be considered as an alternative to myringoplasty.
将不同浓度和分子量的透明质酸制剂局部应用于大鼠实验性鼓膜穿孔，以阐明其改善愈合模式的能力。穿孔位于鼓膜的上后象限。通过耳显微镜检查和组织学切片观察愈合鼓膜的愈合速率和质量变化。透明质酸提高了愈合速率，减少了瘢痕区域的不透明度和结构改变。鼓膜穿孔的闭合时间与透明质酸的浓度相关，而与其分子量或粘度无关。无论其流变学性质如何，在有透明质酸的情况下瘢痕质量均得到改善。透明质酸改善了纤维结缔组织层的修复。高浓度透明质酸治疗人类鼓膜穿孔应被视为鼓膜成形术的替代方法。
