Mastrogiovanni Mauricio, Trostchansky Andres, Rubbo Homero
Departamento de Bioquímica, Facultad de Medicina and Centro de Investigaciones Biomédicas (CEINBIO), Universidad de La República, Montevideo, Uruguay.
Data Brief. 2019 Dec 20;28:105037. doi: 10.1016/j.dib.2019.105037. eCollection 2020 Feb.
Under physiological and pathophysiological conditions, lipid nitration occurs generating nitro-fatty acids (NFA) with pleiotropic activities as modulation of inflammatory cell responses. Foam cell formation and atherosclerotic lesion development have been extensively related to low-density lipoprotein (LDL) oxidation. Considering our manuscript "Fatty acid nitration in human low-density lipoprotein" (https://doi.org/10.1016/j.abb.2019.108190), herein we report the oxidation versus nitration of human LDL protein and lipid fractions. Data is shown on LDL fatty acid nitration, in particular, formation and quantitation of nitro-conjugated linoleic acid (NO-cLA) under mild nitration conditions. In parallel to NO-cLA formation, depletion of endogenous antioxidants, protein tyrosine nitration, and carbonyl formation is observed. Overall, our data propose the formation of a potential anti-atherogenic form of LDL carrying NFA.
在生理和病理生理条件下，会发生脂质硝化反应，生成具有多效活性的硝基脂肪酸（NFA），如对炎症细胞反应的调节。泡沫细胞形成和动脉粥样硬化病变发展与低密度脂蛋白（LDL）氧化密切相关。鉴于我们的论文《人低密度脂蛋白中的脂肪酸硝化》（https://doi.org/10.1016/j.abb.2019.108190），在此我们报告人LDL蛋白和脂质组分的氧化与硝化情况。展示了关于LDL脂肪酸硝化的数据，特别是在温和硝化条件下硝基共轭亚油酸（NO-cLA）的形成和定量。与NO-cLA形成同时，观察到内源性抗氧化剂的消耗、蛋白质酪氨酸硝化和羰基形成。总体而言，我们的数据表明形成了一种携带NFA的潜在抗动脉粥样硬化形式的LDL。
