School of Bioengineering , Dalian University of Technology , Dalian 116024 , China.
Institute of Plant Protection , Chinese Academy of Agricultural Sciences , Beijing 100193 , China.
J Agric Food Chem. 2020 Jan 29;68(4):982-988. doi: 10.1021/acs.jafc.9b06959. Epub 2020 Jan 21.
Cycloxaprid (CYC) is effective in the control of hemipteran pests, but its bioactivity against lepidopteran pests is still unclear. Here, the bioactivity of CYC against lepidopteran pests was found to be much worse than that against hemipteran insects. To reveal the mechanism, the transcriptomes of CYC-treated and untreated larvae were compared. Among the top 20 differentially expressed genes, 11 encode proteins involved in cuticle formation, while only one encodes a detoxifying enzyme. Thus, the cuticle appears to be important for the insensitivity of to CYC. A pretreatment of larvae with methoprene enhanced the bioactivity of CYC by 1.12-fold. Moreover, mixtures of CYC with graphene oxide increased the bioactivity of CYC by 1.88-fold. Because lepidopteran and hemipteran insects often harm crops at the same time, the work can help make full use of CYC and reduce the environmental impacts of using multiple pesticides.
环丙烯（CYC）在防治半翅目害虫方面效果显著，但它对鳞翅目害虫的生物活性尚不清楚。本研究发现，CYC 对鳞翅目害虫的生物活性远不如对半翅目昆虫的活性。为了揭示其机制，比较了 CYC 处理和未处理幼虫的转录组。在 top 20 差异表达基因中，有 11 个编码与表皮形成有关的蛋白，而只有一个编码解毒酶。因此，表皮似乎是 对 CYC 不敏感的重要原因。用灭幼脲预处理幼虫可使 CYC 的生物活性提高 1.12 倍。此外，将 CYC 与氧化石墨烯混合可使 CYC 的生物活性提高 1.88 倍。由于鳞翅目和半翅目昆虫经常同时危害作物，这项工作有助于充分利用 CYC 并减少使用多种杀虫剂对环境的影响。
