Roşu Gabriela Camelia, Pirici Ionica, Istrate-Ofiţeru Anca Maria, Iovan Larisa, Tudorică Valerica, Mogoantă Laurenţiu, Gîlceavă Ion Cristian, Pirici Daniel
Department of Research Methodology, University of Medicine and Pharmacy of Craiova, Romania;
Rom J Morphol Embryol. 2019;60(3):823-830.
Ischemic stroke occurs through embolic or thrombotic obliteration of an artery from cerebral circulation and represents over 80% of all stroke cases. One of the fiercest complications after stroke is edema, which results from imbalanced water diffusion around the blood vessels walls. Water diffusion around blood vessel walls occurs physiologically mainly through two protein-formed pores, namely aquaporins (AQPs) 1 and 4. Here, we compare for the first time the expression patterns and colocalization degrees of the two AQPs in control brain tissue and in peri-ischemic regions, on tissue obtained from eight patients with confirmed ischemic pathology and from five control cases. Our analysis showed that AQP4 is more abundant that AQP1, especially in the cortex and in the organized scar areas. The colocalization of the two markers was high, both located on the astrocytes membranes, but the colocalization degree decreased in the scar peri-ischemic regions. Colocalization with basement membranes was also lower for AQP1 compared to AQP4, in all regions analyzed.
缺血性中风是由于脑循环中动脉的栓塞或血栓形成闭塞所致，占所有中风病例的80%以上。中风后最严重的并发症之一是水肿，它是由血管壁周围水扩散失衡引起的。血管壁周围的水扩散在生理上主要通过两个由蛋白质形成的孔，即水通道蛋白（AQP）1和4进行。在此，我们首次比较了这两种水通道蛋白在确诊为缺血性病变的8例患者和5例对照病例的脑组织及缺血周围区域中的表达模式和共定位程度。我们的分析表明，AQP4比AQP1更丰富，尤其是在皮质和有组织的瘢痕区域。这两种标志物的共定位程度很高，都位于星形胶质细胞膜上，但在缺血周围瘢痕区域共定位程度降低。在所有分析区域中，与基底膜的共定位程度AQP1也低于AQP4。
