糖苷酶对循环中大鼠红细胞存活的影响。

The effect of glycosidases on the survival of rat erythrocytes in circulation.

作者信息

Gutowski K A, Linseman D A, Aminoff D

机构信息

Institute of Gerontology, University of Michigan, Ann Arbor 48109.

出版信息

Carbohydr Res. 1988 Jul 15;178:307-13. doi: 10.1016/0008-6215(88)80120-4.

DOI:10.1016/0008-6215(88)80120-4

PMID:3191511

Abstract

Enzymatic removal of sialyl groups from mammalian erythrocytes resulted in their rapid sequestration from circulation subsequent to autologous transfusion. It has been demonstrated by many investigators that the terminal beta-D-galactosyl group, exposed on red blood cell by in vitro desialosylation, is recognized by an autoimmune anti-galactosyl IgG and/or by a lectin-like receptor on monocytes and macrophages. It is demonstrated herein that the disaccharide structure beta-D-Galp-(1----3)-D-GalpNAc (a) is masked in normal rat RBC, but exposed in asialo-RBC; (b) could be detected with fluorescently-labeled peanut agglutinin; (c) could be released from the asialo-RBC with an endo-N-acetyl-alpha-D-galactosaminidase; and (d) upon its removal by treatment with the endo-N-acetyl-alpha-D-galactosaminidase, enhances the survival of the asialo-RBC in circulation.

摘要

从哺乳动物红细胞中酶促去除唾液酸基团会导致其在自体输血后迅速从循环中被隔离。许多研究者已证明，体外去唾液酸化后红细胞表面暴露的末端β-D-半乳糖基会被自身免疫性抗半乳糖基IgG和/或单核细胞及巨噬细胞上的凝集素样受体识别。本文证明：（a）二糖结构β-D-Galp-(1→3)-D-GalpNAc在正常大鼠红细胞中被掩盖，但在去唾液酸红细胞中暴露；（b）可用荧光标记的花生凝集素检测到；（c）可用内切-N-乙酰-α-D-半乳糖胺酶从去唾液酸红细胞中释放出来；（d）经内切-N-乙酰-α-D-半乳糖胺酶处理将其去除后，可提高去唾液酸红细胞在循环中的存活率。