Department of Orthopedic Surgery, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, No. 111 Dade Road, Guangzhou, Guangdong 510120, China.
Guangzhou University of Chinese Medicine, No. 12, Jichang Road, Baiyun District, Guangzhou 510405, China.
Oxid Med Cell Longev. 2019 Dec 12;2019:6493081. doi: 10.1155/2019/6493081. eCollection 2019.
Spinal cord injury (SCI) has always been considered to be a devastating problem that results in catastrophic dysfunction, high disability rate, low mortality rate, and huge cost for the patient. Stem cell-based therapy, especially using bone marrow mesenchymal stem cells (BMSCs), is a promising strategy for the treatment of SCI. However, SCI results in low rates of cell survival and a poor microenvironment, which limits the therapeutic efficiency of BMSC transplantation. Coenzyme Q10 (CoQ10) is known as a powerful antioxidant, which inhibits lipid peroxidation and scavenges free radicals, and its combined effect with BMSC transplantation has been shown to have a powerful impact on protecting the vitality of cells, as well as antioxidant and antiapoptotic compounds in SCI. Therefore, we aimed to evaluate whether CoQ10 could decrease oxidative stress against the apoptosis of BMSCs and explored its molecular mechanisms. Furthermore, we investigated the protective effect of CoQ10 combined with BMSCs transplanted into a SCI model to verify its ability. Our results demonstrate that CoQ10 treatment significantly decreases the expression of the proapoptotic proteins Bax and Caspase-3, as shown through TUNEL-positive staining and the products of oxidative stress (ROS), while increasing the expression of the antiapoptotic protein Bcl-2 and the products of antioxidation, such as (GSH), against apoptosis and oxidative stress, in a HO-induced model. We also identified consistent results from the CoQ10 treatment of BMSCs transplanted into SCI rats . Moreover, the Nrf-2 signaling pathway was also investigated in order to detail its molecular mechanism, and the results show that it plays an important role, both and . Thus, CoQ10 exerts an antiapoptotic and antioxidant effect, as well as improves the microenvironment and . It may also protect BMSCs from oxidative stress and enhance their therapeutic efficiency when transplanted for SCI treatment.
脊髓损伤 (SCI) 一直被认为是一种毁灭性的问题，会导致灾难性的功能障碍、高残疾率、低死亡率和患者的巨大医疗费用。基于干细胞的治疗，特别是使用骨髓间充质干细胞 (BMSCs)，是治疗 SCI 的一种很有前途的策略。然而，SCI 导致细胞存活率低和微环境差，限制了 BMSC 移植的治疗效果。辅酶 Q10 (CoQ10) 是一种强大的抗氧化剂，可抑制脂质过氧化并清除自由基，其与 BMSC 移植的联合作用已被证明对保护细胞活力以及 SCI 中的抗氧化和抗细胞凋亡化合物具有强大的影响。因此，我们旨在评估 CoQ10 是否可以降低氧化应激对 BMSCs 凋亡的影响，并探讨其分子机制。此外，我们研究了 CoQ10 与移植到 SCI 模型中的 BMSCs 联合使用的保护作用，以验证其能力。我们的结果表明，CoQ10 处理显著降低了促凋亡蛋白 Bax 和 Caspase-3 的表达，如 TUNEL 阳性染色和氧化应激产物 (ROS) 所示，同时增加了抗凋亡蛋白 Bcl-2 的表达和抗氧化产物，如 (GSH)，以对抗凋亡和氧化应激，在 HO 诱导的模型中。我们还从 CoQ10 处理移植到 SCI 大鼠的 BMSCs 中得到了一致的结果。此外，还研究了 Nrf-2 信号通路，以详细了解其分子机制，结果表明它发挥了重要作用，既可以保护细胞免受氧化应激，又可以提高微环境的稳定性和改善细胞功能。它还可能保护 BMSCs 免受氧化应激，并增强其在治疗 SCI 时的治疗效果。
Zotero 插件
