针对人基质细胞衍生因子-2的大鼠单克隆抗体的产生
Generation of Rat Monoclonal Antibodies Specific for Human Stromal Cell-Derived Factor-2.
作者信息
Tanaka Masako, Shiota Masayuki, Koyama Masaru, Nakayama Jun, Yashiro Masakazu, Semba Kentaro, Goda Nobuhito
机构信息
Waseda Institute for Advanced Study, Waseda University, Tokyo, Japan.
Research Support Platform, Osaka City University Graduate School of Medicine, Osaka, Japan.
出版信息
Monoclon Antib Immunodiagn Immunother. 2020 Feb;39(1):23-26. doi: 10.1089/mab.2019.0043. Epub 2020 Jan 9.
Stromal cell-derived factor-2 (SDF-2) is reportedly involved in multiple endoplasmic reticulum (ER) functions, including the misfolded protein catabolic process, protein glycosylation, and ER protein quality control. However, the precise molecular and cellular functions of SDF-2 remain unknown. Previously, we discovered that SDF-2 mediates acquired resistance to oxaliplatin in human gastric cancer cells. In this study, we have generated SDF-2-specific monoclonal antibodies (mAbs), using the rat medial iliac lymph node method, as a tool to explore novel mechanisms of oxaliplatin resistance. The antibodies detected endogenous human SDF-2 in immunoblotting analyses. In addition, immunoprecipitation analyses revealed the availability of these antibodies for human SDF-2. Thus, these mAbs will be available to elucidate molecular and cellular functions of SDF-2 in cancer cells.
据报道，基质细胞衍生因子-2（SDF-2）参与多种内质网（ER）功能，包括错误折叠蛋白的分解代谢过程、蛋白质糖基化以及内质网蛋白质质量控制。然而，SDF-2的确切分子和细胞功能仍不清楚。此前，我们发现SDF-2介导人胃癌细胞对奥沙利铂的获得性耐药。在本研究中，我们使用大鼠髂内侧淋巴结法制备了SDF-2特异性单克隆抗体（mAb），作为探索奥沙利铂耐药新机制的工具。这些抗体在免疫印迹分析中检测到内源性人SDF-2。此外，免疫沉淀分析表明这些抗体可用于检测人SDF-2。因此，这些单克隆抗体将有助于阐明SDF-2在癌细胞中的分子和细胞功能。
Zotero 插件