一种利用大鼠髂内侧淋巴结细胞制备产生大鼠单克隆抗体杂交瘤的新方法。

A novel method of preparing rat-monoclonal antibody-producing hybridomas by using rat medial iliac lymph node cells.

作者信息

Kishiro Y, Kagawa M, Naito I, Sado Y

机构信息

Division of Immunology, Shigei Medical Research Institute, Okayama, Japan.

出版信息

Cell Struct Funct. 1995 Apr;20(2):151-6. doi: 10.1247/csf.20.151.

DOI:10.1247/csf.20.151

PMID:7641297

Abstract

A novel method of preparing hybridomas producing rat monoclonal antibodies was established. The enlarged medial iliac lymph nodes from rats injected via hind footpads with an emulsion of antigen and Freund's adjuvant were used for cell fusion. Ovalbumin was used as a representative antigen. The incidence of hybridomas producing antibody of interest with this method was about 10 times higher than that of hybridomas with the conventional method using mouse or rat spleen cells. The average percentages of hybridomas producing IgG1, IgG2a, IgG2b and IgG2c were 37.1%, 47.0%, 15.9% and 0.0%, respectively. A single injection with antigen was sufficient for immunization in this method.

摘要

建立了一种制备产生大鼠单克隆抗体的杂交瘤的新方法。将通过后足垫注射抗原与弗氏佐剂乳剂的大鼠的肿大的髂内侧淋巴结用于细胞融合。卵清蛋白用作代表性抗原。用这种方法产生感兴趣抗体的杂交瘤的发生率比使用小鼠或大鼠脾细胞的传统方法产生的杂交瘤高约10倍。产生IgG1、IgG2a、IgG2b和IgG2c的杂交瘤的平均百分比分别为37.1%、47.0%、15.9%和0.0%。在这种方法中，单次注射抗原就足以进行免疫。