College of Science, Beijing University of Chemical Technology, Beijing, 100029, China.
Beijing Key Laboratory of Environmentally Harmful Chemical Analysis, Beijing University of Chemical Technology, Beijing, 100029, China.
J Mass Spectrom. 2020 May;55(5):e4499. doi: 10.1002/jms.4499. Epub 2020 Feb 18.
The supercritical fluid chromatography coupled with mass spectrometry (SFC-MS) method and liquid chromatography coupled with mass spectrometry (LC-MS) method were developed for the separation and characterization of poly (ethylene oxide) methyl glucose sesquistearate (PEO-Glu-sesquistearate). The products of PEO-Glu-sesquistearate are composed of complex oligomers. The relationship between molecular structure of these oligomers and chromatographic retention behavior in both SFC and LC were discussed and compared. As compared with LC, hydrophobic moieties of compounds favor the fast elution in SFC. The different series can be better separated by LC, while the homologues compounds in same series can be better separated by SFC, and SFC-MS provided more comprehensive structural information. Different series such as PEO-distearate, PEO-stearate, PEO, PEO-Glu-tetrastearate, PEO-Glu-tristearate, PEO-Glu-distearate, PEO-Glu-stearate, and PEO-Glu were identified by MS/MS.
建立了超临界流体色谱-质谱联用（SFC-MS）和液相色谱-质谱联用（LC-MS）方法，用于聚（氧化乙烯）甲基葡萄糖倍半硬脂酸酯（PEO-Glu-sesquistearate）的分离和结构鉴定。PEO-Glu-sesquistearate 的产物由复杂的低聚物组成。讨论并比较了这些低聚物的分子结构与 SFC 和 LC 中色谱保留行为之间的关系。与 LC 相比，化合物的疏水性部分有利于 SFC 中的快速洗脱。不同系列可以通过 LC 更好地分离，而同一系列中的同系物可以通过 SFC 更好地分离，并且 SFC-MS 提供了更全面的结构信息。通过 MS/MS 鉴定了不同的系列，如 PEO-硬脂酸酯、PEO-硬脂酸酯、PEO、PEO-Glu-四硬脂酸酯、PEO-Glu-三硬脂酸酯、PEO-Glu-二硬脂酸酯、PEO-Glu-硬脂酸酯和 PEO-Glu。
