Tripathi Nagesh K, Shrivastava Ambuj
Bioprocess Scale Up Facility, Defence Research and Development Establishment, Gwalior, India.
Division of Virology, Defence Research and Development Establishment, Gwalior, India.
Front Bioeng Biotechnol. 2019 Dec 20;7:420. doi: 10.3389/fbioe.2019.00420. eCollection 2019.
Infectious diseases, along with cancers, are among the main causes of death among humans worldwide. The production of therapeutic proteins for treating diseases at large scale for millions of individuals is one of the essential needs of mankind. Recent progress in the area of recombinant DNA technologies has paved the way to producing recombinant proteins that can be used as therapeutics, vaccines, and diagnostic reagents. Recombinant proteins for these applications are mainly produced using prokaryotic and eukaryotic expression host systems such as mammalian cells, bacteria, yeast, insect cells, and transgenic plants at laboratory scale as well as in large-scale settings. The development of efficient bioprocessing strategies is crucial for industrial production of recombinant proteins of therapeutic and prophylactic importance. Recently, advances have been made in the various areas of bioprocessing and are being utilized to develop effective processes for producing recombinant proteins. These include the use of high-throughput devices for effective bioprocess optimization and of disposable systems, continuous upstream processing, continuous chromatography, integrated continuous bioprocessing, Quality by Design, and process analytical technologies to achieve quality product with higher yield. This review summarizes recent developments in the bioprocessing of recombinant proteins, including in various expression systems, bioprocess development, and the upstream and downstream processing of recombinant proteins.
传染病与癌症一样，是全球人类主要的死因之一。为数百万人大规模生产用于治疗疾病的治疗性蛋白质是人类的基本需求之一。重组DNA技术领域的最新进展为生产可作为治疗剂、疫苗和诊断试剂的重组蛋白铺平了道路。用于这些应用的重组蛋白主要是在实验室规模以及大规模环境中，使用原核和真核表达宿主系统生产的，如哺乳动物细胞、细菌、酵母、昆虫细胞和转基因植物。高效生物加工策略的发展对于具有治疗和预防重要性的重组蛋白的工业生产至关重要。最近，生物加工的各个领域都取得了进展，并正被用于开发生产重组蛋白的有效工艺。这些进展包括使用高通量设备进行有效的生物工艺优化，以及使用一次性系统、连续上游加工、连续色谱、集成连续生物加工、质量源于设计和过程分析技术，以实现更高产量的优质产品。本综述总结了重组蛋白生物加工的最新进展，包括在各种表达系统、生物工艺开发以及重组蛋白的上游和下游加工方面的进展。
