Dutton J E, Rogers L J
Biochim Biophys Acta. 1978 Dec 20;537(2):501-6. doi: 10.1016/0005-2795(78)90535-4.
Isoelectric points of ferredoxins, flavodoxins and a rubredoxin from a range of sources were measured by electrofocusing over the pH range between 2.5 and 5.0 on thin layers of polyacrylamide gel. The pH gradient along the gel was measured directly by a surface electrode. The isoelectric points of the plant-type ferredoxins were between approx. 3.15 and 3.35, and those of the flavodoxins close to 3.5. Ferredoxin, rubredoxin and flavodoxin from Clostridium pasteurianum had isolectric points of the of 2.75, 2.9, and 3.1, respectively. The values for the isoelectric points ferredoxins are significantly lower than previous results in the literature suggest.
通过在pH值范围为2.5至5.0的聚丙烯酰胺凝胶薄层上进行电聚焦，测定了来自一系列来源的铁氧化还原蛋白、黄素氧化还原蛋白和一种红素氧还蛋白的等电点。沿凝胶的pH梯度通过表面电极直接测量。植物型铁氧化还原蛋白的等电点约在3.15至3.35之间，黄素氧化还原蛋白的等电点接近3.5。巴氏梭菌的铁氧化还原蛋白、红素氧还蛋白和黄素氧化还原蛋白的等电点分别为2.75、2.9和3.1。铁氧化还原蛋白等电点的值明显低于文献中先前的结果。
