Materials Science and Engineering College, Guilin University of Technology, Guilin, China.
Dental Clinic and Experimental Center of Medical Sciences, Guilin Medical University, Guilin, China.
J Biomater Sci Polym Ed. 2020 Apr;31(6):729-746. doi: 10.1080/09205063.2020.1714534. Epub 2020 Jan 25.
A new bi-component poly(vinylalcohol)(PVA)/chitosan(CS)-poly(e-caprolactone)(PCL)/gelatin(Gel) multiscale electrospun scaffold was developed and analyzed in comparison with several other single scale systems. To mimic the native extracellular matrix in composition and structure and promote the migration of cells inside the scaffold, PVA/CS composite nanofibers (102 ± 52 nm) and PCL/Gelcomposite microfiber (2.5 ± 1.0 µm) were simultaneously electrospun from the two opposite syringes and mixed on a rotating mandrel to generate a bi-component multi-scale membrane. The bi-component membrane was crosslinked by glutaraldehyde vapor to maintain its fiber morphology in the wet stage. Morphology, shrinkage and spectroscopic of the electrospun membranes were characterized. To test the newly developed multiscale membrane, we seeded mesenchymal stem cells (MSCs) derived from rabbit onto five different fiber scaffolds (PVA, PVA/CS, PCL, PCL/Gel and PVA/CS-PCL/Gel) and compared cell adhesion and proliferation between different groups for 3 days using scanning electron microscopy, inverted microscope observations assay and MTT colorimetric. Cell culture results suggest that the incorporation of chitosan and gelatin could enhance cell adhesion and cell spreading in comparison to the performance of single component scaffolds of PVA and PCL. The multiscale PVA/CS-PCL/Gel membrane scaffolds provide a better environment to increase the growth, adhesion, and proliferation of cells. Scanning electron microscopy (SEM) observations showed that the cells were not only adhered well and proliferated on the surface of the scaffolds, but were also able to infiltrate inside the scaffold within 3 days of culture. MTT assay and inverted microscope observations also showed that the PVA/CS-PCL/Gel complex fibrous membrane exhibited better activity than other single component/scale systems scaffolds. Our results provide the underlying insights needed to guide the design of the native extracellular matrix.
一种新型双组分聚（聚乙烯醇）（PVA）/壳聚糖（CS）-聚（己内酯）（PCL）/明胶（Gel）多尺度电纺支架被开发出来，并与其他几种单尺度系统进行了比较分析。为了在组成和结构上模拟天然细胞外基质，并促进细胞在支架内的迁移，从两个相反的注射器中同时电纺出 PVA/CS 复合纳米纤维（102±52nm）和 PCL/Gel 复合微纤维（2.5±1.0μm），并在旋转芯轴上混合以生成双组分多尺度膜。双组分膜通过戊二醛蒸气交联以在湿态下保持其纤维形态。对电纺膜的形态、收缩和光谱进行了表征。为了测试新开发的多尺度膜，我们将兔来源的间充质干细胞（MSCs）接种到五种不同的纤维支架（PVA、PVA/CS、PCL、PCL/Gel 和 PVA/CS-PCL/Gel）上，并通过扫描电子显微镜、倒置显微镜观察实验和 MTT 比色法比较不同组别的细胞粘附和增殖情况，共 3 天。细胞培养结果表明，与 PVA 和 PCL 单一组分支架相比，壳聚糖和明胶的掺入可以增强细胞的粘附和细胞扩展。多尺度 PVA/CS-PCL/Gel 膜支架提供了更好的环境，可以促进细胞的生长、粘附和增殖。扫描电子显微镜（SEM）观察表明，细胞不仅在支架表面良好地粘附和增殖，而且在培养 3 天后还能够渗透到支架内部。MTT 测定和倒置显微镜观察也表明，PVA/CS-PCL/Gel 复合纤维膜比其他单一组分/尺度系统支架具有更好的活性。我们的结果为指导设计天然细胞外基质提供了必要的深入了解。
