Kato N, Sahm H, Schütte H, Wagner F
Biochim Biophys Acta. 1979 Jan 12;566(1):1-11. doi: 10.1016/0005-2744(79)90242-0.
Glucose-6-phosphate dehydrogenase (D-glucose-6-phosphate: NADP oxidoreductase, EC 1.1.1.49) and 6-phosphogluconate dehydrogenase (6-phospho-D-gluconate: NADP oxidoreductase, EC 1.1.1.44) were purified approx. 1700 fold and 330 fold, respectively, from Candida boidinii grown on methanol. The final enzyme preparations were homogeneous as judged by polyacrylamide gel electrophoresis. The molecular weights of the enzymes were estimated to be 118 000 and 110 000, respectively. Both enzymes are composed of two probably identical subunits and the molecular weights of the polypeptide chains were calculated to be 61 000 and 58 000, respectively. From a consideration of enzyme activities and types of inhibition by different metabolites the role of these two enzymes in glucose- and methanol-metabolism is discussed.
从以甲醇为碳源生长的博伊丁假丝酵母中分别纯化出葡萄糖-6-磷酸脱氢酶（D-葡萄糖-6-磷酸：NADP氧化还原酶，EC 1.1.1.49）和6-磷酸葡萄糖酸脱氢酶（6-磷酸-D-葡萄糖酸：NADP氧化还原酶，EC 1.1.1.44），纯化倍数分别约为1700倍和330倍。通过聚丙烯酰胺凝胶电泳判断，最终的酶制剂是均一的。这两种酶的分子量估计分别为118000和110000。两种酶均由两个可能相同的亚基组成，多肽链的分子量经计算分别为61000和58000。通过考虑酶活性以及不同代谢物的抑制类型，讨论了这两种酶在葡萄糖和甲醇代谢中的作用。
