单卵双胞胎选择性胎儿生长受限中PHLDA2和IGF2的胎盘表达
Placental expression of PHLDA2 and IGF2 in selective intrauterine growth restriction in monozygotic twins.
作者信息
Chen Jie, Pan Shilei, Hang Lilin, Zhong Mei, Yu Yanhong
机构信息
Zhujiang Hospital, Southern Medical University Guangzhou 510280, China.
Department of Obstetrics and Gynecology, Nanfang Hospital, Southern Medical University Guangzhou 510515, China.
出版信息
Int J Clin Exp Pathol. 2018 Feb 1;11(2):876-881. eCollection 2018.
OBJECTIVE
To compare the expression of imprinted genes PHLDA2 and IGF2 in the peripheral blood, placenta and fetal umbilical cord blood from normal single chorionic twins (MCDA) and single chorionic twins combined with sIUGR, in order to explore the pathogenesis relationship between the expression of PHLDA2 and IGF2 with single chorionic twins combined with sIUGR.
METHODS
Immunohistochemical method was applied to detect the expression of PHLDA2 and IGF2 in maternal placenta of MCDA and normal MCDA. ELISA method was applied to detect the expression of PHLDA2 and IGF2 in two groups of umbilical cord blood.
RESULTS
There was a significant difference in two groups of maternal age and neonatal birth weight (P<0.05). Immunohistochemical results: IGF2 in the normal MCDA maternal placenta was high expression. The number of IGF2-positive cells in MCDA-sIUGR group A1 decreased slightly and the staining decreased slightly. The number of IGF2-positive cells in A2 group was significantly decreased, and the staining was weakened. In normal MCDA placenta, PHLDA2 cells were colorless, or occasionally in pale yellow; the number of PHLDA2 positive cells in MCDA sIUGR A1 group was slightly increased, the staining was light yellow or colorless. In A2 group, the number of positive cells increased significantly, the staining was brown or brownish-yellow. ELISA results showed that there were significant differences in the expression of IGF2 and PHLDA2 between cord blood of MCDA sIUGR and neonatal cord blood (P<0.05). The expression of IGF2 in MCDA sIUGR and normal MCDA twins were also significantly different (P<0.01).
CONCLUSION
The differential expression of PHLDA2 and IGF2 may be one of the causes of selective intrauterine growth restriction.
目的
比较正常单绒毛膜双胎（MCDA）及合并选择性胎儿生长受限（sIUGR）的单绒毛膜双胎外周血、胎盘及胎儿脐带血中印迹基因PHLDA2和IGF2的表达，以探讨PHLDA2和IGF2表达与单绒毛膜双胎合并sIUGR发病机制的关系。
方法
采用免疫组织化学方法检测MCDA及正常MCDA孕妇胎盘组织中PHLDA2和IGF2的表达。采用酶联免疫吸附测定（ELISA）法检测两组脐带血中PHLDA2和IGF2的表达。
结果
两组孕妇年龄及新生儿出生体重比较，差异有统计学意义（P<0.05）。免疫组织化学结果显示：正常MCDA孕妇胎盘组织中IGF2呈高表达。MCDA-sIUGR组A1中IGF2阳性细胞数量略有减少，染色略有减弱。A2组IGF2阳性细胞数量明显减少，染色减弱。正常MCDA胎盘组织中PHLDA2细胞不着色，或偶尔呈淡黄色；MCDA sIUGR A1组PHLDA2阳性细胞数量略有增加，染色呈淡黄色或不着色。A2组阳性细胞数量明显增加，染色呈棕色或棕黄色。ELISA结果显示，MCDA sIUGR脐带血与新生儿脐带血中IGF2和PHLDA2表达差异有统计学意义（P<0.05）。MCDA sIUGR与正常MCDA双胎中IGF2表达也有显著差异（P<0.01）。
结论
PHLDA2和IGF2的差异表达可能是选择性胎儿生长受限的原因之一。
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