He Zongze, Li Jijun, Mei Yan, Lv Yang, Zhu Fei, Liu Ran, Wang Xu, Yao Fenghua, Zhang Li, Chen Xiangmei
Department of Nephrology, Chinese PLA General Hospital, Chinese PLA Institute of Nephrology, State key Laboratory of Kidney Diseases, National Clinical Research Center for Kidney Diseases Beijing 100853, China.
The First Hospital Affiliated to The Chinese PLA General Hospital Beijing, China.
Int J Clin Exp Pathol. 2018 Feb 1;11(2):1054-1061. eCollection 2018.
To investigate the role of toll like receptors (TLRs) 2 and 4 in rhabdomyolysis (RM)-related acute kidney injury (AKI). Wild-type (WT) mice and TLR2 knockout (TLR2) or TLR4 knockout (TLR4) mice were injected with either saline (sham) or glycerin (to induce RM-related AKI). Samples were collected for detection of 0 h 24 h (Cr) creatinine, urea nitrogen (BUN), creatine kinase (CK), and PAS staining of renal tissues. Serum Cr and BUN level was significantly increased in TLR2 and TLR4AKI groups more than those in the control group and the WT mice in AKI group. TLR4AKI group Cr, BUN level, and the pathological damage was lightest. The expression levels of signal transduction proteins in TLR2 and TLR4AKI group were higher than in the control group, but was lower than that in the wild AKI group, with the TLR4AKI group having the lowest levels. The expression level of inflammatory factor mRNA in TLR2 and TLR4AKI groups was higher than that in control group, but was lower than that in wild AKI group, with TLR4AKI group displaying lowest levels. Knockout of TLRs 2 and 4 decreased kidney inflammation and improved RM-related AKI.
为研究Toll样受体（TLRs）2和4在横纹肌溶解症（RM）相关急性肾损伤（AKI）中的作用。将野生型（WT）小鼠和TLR2基因敲除（TLR2）或TLR4基因敲除（TLR4）小鼠分别注射生理盐水（假手术）或甘油（诱导RM相关AKI）。采集样本检测0小时、24小时的肌酐（Cr）、尿素氮（BUN）、肌酸激酶（CK），并对肾组织进行PAS染色。TLR2和TLR4 AKI组的血清Cr和BUN水平显著高于对照组和AKI组中的WT小鼠。TLR4 AKI组的Cr、BUN水平及病理损伤最轻。TLR2和TLR4 AKI组信号转导蛋白的表达水平高于对照组，但低于野生型AKI组，其中TLR4 AKI组水平最低。TLR2和TLR4基因敲除可减轻肾脏炎症并改善RM相关AKI。
