小鼠胚胎衍生干细胞中原癌基因int-2的破坏：一种将突变靶向非选择基因的通用策略。

Disruption of the proto-oncogene int-2 in mouse embryo-derived stem cells: a general strategy for targeting mutations to non-selectable genes.

作者信息

Mansour S L, Thomas K R, Capecchi M R

机构信息

Howard Hughes Medical Institute, Department of Biology, University of Utah, Salt Lake City 84112.

出版信息

Nature. 1988 Nov 24;336(6197):348-52. doi: 10.1038/336348a0.

DOI:10.1038/336348a0

PMID:3194019

Abstract

Gene targeting--homologous recombination of DNA sequences residing in the chromosome with newly introduced DNA sequences--in mouse embryo-derived stem cells promises to provide a means to generate mice of any desired genotype. We describe a positive nd negative selection procedure that enriches 2,000-fold for those cells that contain a targeted mutation. The procedure was applied to the isolation of hprt- and int-2- mutants, but it should be applicable to any gene.

摘要

基因打靶——即位于染色体上的DNA序列与新导入的DNA序列进行同源重组——在源自小鼠胚胎的干细胞中有望提供一种手段来培育出具有任何所需基因型的小鼠。我们描述了一种正负选择程序，该程序可使含有靶向突变的细胞富集2000倍。该程序已应用于分离次黄嘌呤磷酸核糖转移酶（hprt）和int-2突变体，但它应该适用于任何基因。