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分子技术是如何从自然系统中发展而来的。

How molecular techniques are developed from natural systems.

机构信息

Charles Perkins Centre and School of Life and Environmental Sciences, The University of Sydney, Sydney 2006, Australia.

Department of Organismic and Evolutionary Biology, Harvard University.

出版信息

Genetics. 2023 Jul 6;224(3). doi: 10.1093/genetics/iyad067.

Abstract

A striking characteristic of the molecular techniques of genetics is that they are derived from natural occurring systems. RNA interference, for example, utilizes a mechanism that evolved in eukaryotes to destroy foreign nucleic acid. Other case studies I highlight are restriction enzymes, DNA sequencing, polymerase chain reaction, gene targeting, fluorescent proteins (such as, green fluorescent protein), induced pluripotent stem cells, and clustered regularly interspaced short palindromic repeats-CRISPR associated 9. The natural systems' strategy for technique development means that biologists utilize the activity of a mechanism's effector (protein or RNA) and exploit biological specificity (protein or nucleic acid can cause precise reactions). I also argue that the developmental trajectory of novel molecular techniques, such as RNA interference, has 4 characteristic phases. The first phase is discovery of a biological phenomenon. The second phase is identification of the biological mechanism's trigger(s): the effector and biological specificity. The third phase is the application of the trigger(s) as a technique. The final phase is the maturation and refinement of the technique. Developing new molecular techniques from nature is crucial for future genetic research.

摘要

遗传学分子技术的一个显著特点是,它们源自自然发生的系统。例如,RNA 干扰利用了真核生物进化而来的一种机制来破坏外来核酸。我强调的其他案例研究包括限制酶、DNA 测序、聚合酶链式反应、基因靶向、荧光蛋白(如绿色荧光蛋白)、诱导多能干细胞和成簇规律间隔短回文重复序列-CRISPR 相关 9。技术开发的自然系统策略意味着生物学家利用机制效应物(蛋白质或 RNA)的活性,并利用生物特异性(蛋白质或核酸可以引起精确的反应)。我还认为,新型分子技术(如 RNA 干扰)的发展轨迹具有 4 个特征阶段。第一阶段是发现生物学现象。第二阶段是确定生物机制的触发因素:效应物和生物特异性。第三阶段是将触发因素(效应物和生物特异性)应用于技术。最后一个阶段是技术的成熟和完善。从自然界中开发新的分子技术对于未来的遗传研究至关重要。

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