Deans J A, Knight A M, Jean W C, Waters A P, Cohen S, Mitchell G H
Department of Immunology, UMDS, Guy's Hospital Medical School, London.
Parasite Immunol. 1988 Sep;10(5):535-52. doi: 10.1111/j.1365-3024.1988.tb00241.x.
A minor Plasmodium knowlesi 66 kD antigen, which plays an essential role in merozoite invasion, has been shown to be stable in distinct variants and strains of the parasite, and in the face of a specific immune response from the host. Parasites were unable to produce novel molecule(s) to replace it functionally, even in the presence of specific immune pressure. Rhesus monkeys immunized with the purified 66 kD antigen, with saponin as adjuvant, produced antibody which inhibited merozoite invasion of red cells in vitro. Four out of six immunized rhesus monkeys demonstrated clinically effective immunity when challenged at a time of known or presumed high inhibitory antibody titre. When immunization failed to protect, it was ascribed to insufficient levels of specific antibody attributable either to a suboptimal dose of antigen or the use of an inadequate adjuvant.
诺氏疟原虫一种分子量为66 kD的次要抗原，在裂殖子入侵过程中发挥着至关重要的作用，已被证明在该寄生虫的不同变体和菌株中以及面对宿主的特异性免疫反应时都很稳定。即使存在特异性免疫压力，寄生虫也无法产生新的分子来在功能上替代它。用纯化的66 kD抗原并以皂苷作为佐剂免疫恒河猴，产生了在体外抑制裂殖子入侵红细胞的抗体。六只免疫的恒河猴中有四只在已知或推测为高抑制性抗体滴度时受到攻击时表现出临床有效的免疫力。当免疫未能起到保护作用时，这归因于特异性抗体水平不足，这要么是由于抗原剂量次优，要么是由于使用了不充分的佐剂。
