Liu Xueqing, Lang Qiaoli, Wu Meng, You Xiaoyan, He Qiling, Luo Ling, Liu Zijia, Xiao Puying, Huang Nan, Yang Xi, Ge Liangpeng
Chongqing Academy of Animal Sciences Chongqing China.
Key Laboratory of Pig Industry Sciences Ministry of Agriculture Chongqing China.
Animal Model Exp Med. 2019 Dec 18;2(4):334-339. doi: 10.1002/ame2.12095. eCollection 2019 Dec.
Fetal bovine serum (FBS) is widely used in cell cultures due to its high stability and easy access. It was also used as a substitute for porcine follicular fluid (PFF) in previous studies. However, FBS components are unclear, and the presence of FBS in culture media may introduce a variation from batch to batch. This study aimed to establish an effective method to screen FBS in place of PFF in the culture media for porcine oocytes in vitro. We screened FBS from different sources by using porcine fetal fibroblast cells. The effects of six FBS samples on porcine fetal fibroblast cell growth were tested via frozen cell survival assay, cell clone formation assay, cell growth curve, and cell passage activity assay. The best serum that we called GFBS (heat-inactivated FBS, cat. no. 10500-64; Gibco) showed a similar effect on the maturation and development of porcine oocytes to that of PFF and can be used as a good substitute for PFF. These results suggested that the porcine fetal fibroblast cell culture test can be used as a valuable method to screen FBS for porcine oocyte maturation and embryonic development in vitro.
胎牛血清（FBS）因其高稳定性和易于获取而被广泛应用于细胞培养。在先前的研究中，它也被用作猪卵泡液（PFF）的替代品。然而，FBS的成分尚不清楚，并且培养基中FBS的存在可能会导致批次间的差异。本研究旨在建立一种有效的方法，用于筛选可在体外猪卵母细胞培养基中替代PFF的FBS。我们通过使用猪胎儿成纤维细胞筛选了不同来源的FBS。通过冻存细胞存活试验、细胞克隆形成试验、细胞生长曲线和细胞传代活性试验，测试了六种FBS样品对猪胎儿成纤维细胞生长的影响。我们称为GFBS（热灭活FBS，货号10500-64；Gibco）的最佳血清对猪卵母细胞的成熟和发育显示出与PFF相似的效果，并且可以用作PFF的良好替代品。这些结果表明，猪胎儿成纤维细胞培养试验可作为一种有价值的方法，用于筛选在体外支持猪卵母细胞成熟和胚胎发育的FBS。
