Center of Precision Analysis and Material Research, National Taipei University of Technology, Taipei, Taiwan, ROC.
Division of Electrical and Electronic Engineering, Manchester Metropolitan University, Chester Road, Manchester, United Kingdom.
Ultrason Sonochem. 2020 May;63:104917. doi: 10.1016/j.ultsonch.2019.104917. Epub 2019 Dec 4.
In this work, the metal oxide and biopolymer nanocomposites on multiwalled carbon nanotubes (MWCNT) were prepared using a simple sonochemical method. The hexagonal nanorods of zinc oxide (ZnO NR) were synthesized by probe sonication (frequency = 20 kHz, amplitude = 50) method and were integrated on ultrasonically functionalized MWCNT-cellulose nanocrystals (MWCNT-CNC) for the first time. The stable hemin bio-composites also were prepared using the bath sonication (37 kHz of frequency, 150 W of power) method, and was used for the selective and ultrasensitive electrochemical detection of HO. The UV-Vis spectroscopy studies confirmed the presence of native hemin on MWCNT-CNC/ZnO NR nanocomposite. Cyclic voltammetry studies revealed that an enhanced redox electrochemical behaviour of hemin was observed on hemin immobilised MWCNT-CNC/ZnO NR nanocomposite than that of other hemin modified electrodes. Also, the MWCNT-CNC/ZnO NR/hemin modified SPCE showed 2.3 folds higher electrocatalytic activity with a lower reduction potential (-0.2 V) towards HO than that of other investigated hemin modified electrodes including hemin/MWCNT and hemin/CNC-ZnO. The fabricated biosensor displayed a stable amperometric response (-0.2 V vs Ag/AgCl) in the linear concentration of HO ranging up to 4183.3 µM with a lower detection limit of 4.0 nM.
在这项工作中，采用简单的超声化学方法制备了金属氧化物和生物聚合物纳米复合材料在多壁碳纳米管（MWCNT）上。通过探针超声（频率= 20 kHz，幅度= 50）法合成了氧化锌（ZnO NR）的六方纳米棒，并首次将其集成在超声功能化的 MWCNT-纤维素纳米晶（MWCNT-CNC）上。还采用浴超声（频率为 37 kHz，功率为 150 W）法制备了稳定的血红素生物复合材料，并用于 HO 的选择性和超灵敏电化学检测。紫外可见光谱研究证实了 MWCNT-CNC/ZnO NR 纳米复合材料中原位血红素的存在。循环伏安研究表明，在血红素固定化 MWCNT-CNC/ZnO NR 纳米复合材料上观察到血红素的氧化还原电化学行为增强，而在其他血红素修饰电极上则观察到增强。此外，MWCNT-CNC/ZnO NR/hemin 修饰的 SPCE 对 HO 的电催化活性比其他研究的血红素修饰电极（包括 hemin/MWCNT 和 hemin/CNC-ZnO）高 2.3 倍，还原电位（-0.2 V）更低。所制备的生物传感器在 HO 的线性浓度范围内（4183.3 µM）显示出稳定的安培响应（相对于 Ag/AgCl 为-0.2 V），检测限低至 4.0 nM。
