Nazari A, Chehelcheraghi F
Bratisl Lek Listy. 2020;121(1):14-21. doi: 10.4149/BLL_2020_003.
Apelin is an active endogenous peptide, which affects blood vessels. Also exercise increases angiogenesis after myocardial infarction and exerts cardio protective effects. The aim of the present study was to investigate the effect of Apelin and aerobic exercise on reducing the severity of Ischemia-reperfusion injury in rats.
The rats were divided into the following 4 groups 8 weeks before surgery (Langendorff model of perfusion): I) Ischemia-reperfusion (I/R), II: Exercise Ischemia- reperfusion (EX+I/R), III: Apelin+Ischemia-reperfusion (APE+I/R) (Apelin 10 nmol/kg/day, i.p), and IV: Exercise+Apelin+Ischemia-reperfusion (EX+APE+I/R). Exercise was performed on a treadmill 8 weeks before the surgery at a speed of 17 m/min for 10 to 50 min/day. The ventricular function was evaluated after I/R injury, histopathological and immunohistopathology indices were then measured at the scar tissue.
The results of H(et)E, Masson's trichrome staining indicated that APE+EX pre-treatment reduced cardiac fibrosis and the percentage of collagen deposition. It also enhanced the microvessels density (MVD) and decreased the number of inflammatory cells and apoptosis rate.
According to our study, Apelin and exercise preconditioning had anti-fibrotic and anti-apoptotic effects on the ischemia-reperfusion myocardium cells, which could lead to the protection of cardiac cells (Tab. 5, Fig. 3, Ref. 33).
Apelin是一种影响血管的活性内源性肽。运动也可增加心肌梗死后的血管生成并发挥心脏保护作用。本研究的目的是探讨Apelin和有氧运动对减轻大鼠缺血再灌注损伤严重程度的影响。
在手术前8周（Langendorff灌注模型）将大鼠分为以下4组：I）缺血再灌注组（I/R），II：运动缺血再灌注组（EX+I/R），III：Apelin+缺血再灌注组（APE+I/R）（Apelin 10 nmol/kg/天，腹腔注射），IV：运动+Apelin+缺血再灌注组（EX+APE+I/R）。在手术前8周，大鼠在跑步机上以17米/分钟的速度运动，每天10至50分钟。在缺血再灌注损伤后评估心室功能，然后在瘢痕组织处测量组织病理学和免疫组织病理学指标。
苏木精-伊红（H&E）、Masson三色染色结果表明，APE+EX预处理可减少心脏纤维化和胶原沉积百分比。它还提高了微血管密度（MVD），减少了炎症细胞数量和凋亡率。
根据我们的研究，Apelin和运动预处理对缺血再灌注心肌细胞具有抗纤维化和抗凋亡作用，这可能导致对心脏细胞的保护（表5，图3，参考文献33）。
