Quantitative distribution and interaction of Zega with host cells in visceral organs of chickens infected orally, intraperitoneally and per cloaca.

Immunohistochemical study of the visceral organs of chickens experimentally infected with Zega by three routes was carried out to compare the quantitative distribution and interaction of the organism with host cells. 100 birds comprising of 2 week-old chickens were divided into 4 groups of 25 each. Group A was inoculated orally, group B intraperitoneally, group C were administered per cloaca and D were not inoculated and served as control. All the infected birds were inoculated with 0.2 ml of 1 × 10 cfu of the bacteria. Two birds from each group were sacrificed every 24 h post infection. Samples of visceral organs were collected for immunohistochemistry. The distribution of Zega in every organ was taken as Mean ± SD of the number of foci of immunoreactions and Compared using a 2-way ANOVA. The interaction of Zega with host cells was determined by taking the percentage of the days post infection in which immunoreactions were detected in host cells in each route of infection. The distribution of the organism was highest in the lung of intraperitoneally infected chickens (83.95 ± 27.89) and lowest in the heart (5.21 ± 3.65) of chickens that were infected per cloaca. The highest percentage of interaction of Zega was recorded in the epithelial (100%) and blood (100%) cells in all the routes of infection. There were variations in the distribution of Zega in visceral organs of chickens but the level of interactions with host cells were similar even when infected through different routes.