Gupta Sanjeev, Jindal Naresh, Khokhar Raj S, Asrani Rajesh K, Ledoux David R, Rottinghaus George E
Department of Veterinary Epidemiology and Preventive Medicine, Chaudhary Charan Singh Haryana Agricultural University, Hisar, India.
Avian Pathol. 2008 Jun;37(3):265-72. doi: 10.1080/03079450802043759.
A study was conducted to evaluate the individual and combined effects of ochratoxin A (OA) and Salmonella enterica serovar Gallinarum (S. Gallinarum) on gross and histopathological changes in broiler chickens. One hundred and seventy-six 1-day-old broiler chicks were divided into two groups of 88 chicks each; one group was fed a control mash diet, and the other group was fed a mash diet containing 2 parts/10(6) OA. On day 14, each group was further subdivided into two groups, with one group inoculated with S. Gallinarum intraperitoneally (1.25 x 10(10) colony-forming units/0.5 ml) whereas the other group was not inoculated with S. Gallinarum. Four birds from each group were sacrificed on 1, 2, 3, 5, 7, 10, 14 and 21 days post inoculation to record pathological changes in different organs. Gross and microscopic changes in OA-fed birds indicated the kidneys and bursa of Fabricius as the primary organs to be affected by this toxin. Gross and microscopic changes due to S. Gallinarum infection indicated the liver and spleen as the primary organs affected by this infection. The effects of OA on the kidney and bursa of Fabricius were enhanced following S. Gallinarum infection. Degenerative changes and interstitial nephritis in the kidneys, and lymphocyte depletion from bursal follicles were more pronounced and were observed earlier in the combination group. In conclusion, data indicate that birds fed OA and infected with S. Gallinarum will demonstrate increased pathology compared with birds fed OA alone or those infected with S. Gallinarum but not fed OA.
进行了一项研究，以评估赭曲霉毒素A（OA）和鸡伤寒沙门氏菌（鸡白痢沙门氏菌）对肉鸡大体和组织病理学变化的单独及联合影响。176只1日龄肉鸡雏鸡被分为两组，每组88只；一组饲喂对照粉料日粮，另一组饲喂含有2份/10⁶ OA的粉料日粮。在第14天，每组进一步细分为两组，一组腹腔接种鸡白痢沙门氏菌（1.25×10¹⁰菌落形成单位/0.5毫升），而另一组不接种鸡白痢沙门氏菌。在接种后1、2、3、5、7、10、14和21天，每组处死4只鸡，以记录不同器官的病理变化。饲喂OA的鸡的大体和微观变化表明，肾脏和法氏囊是受这种毒素影响的主要器官。鸡白痢沙门氏菌感染引起的大体和微观变化表明，肝脏和脾脏是受这种感染影响的主要器官。鸡白痢沙门氏菌感染后，OA对肾脏和法氏囊的影响增强。联合组中，肾脏的退行性变化和间质性肾炎以及法氏囊滤泡中的淋巴细胞耗竭更为明显，且出现得更早。总之，数据表明，与单独饲喂OA的鸡或感染鸡白痢沙门氏菌但未饲喂OA的鸡相比，饲喂OA并感染鸡白痢沙门氏菌的鸡将表现出更严重的病理学变化。
