将DNA显微注射到三带喙库蚊卵中并检测整合情况。

Microinjection of DNA into Aedes triseriatus ova and detection of integration.

作者信息

McGrane V, Carlson J O, Miller B R, Beaty B J

机构信息

Department of Microbiology, Colorado State University, Fort Collins 80523.

出版信息

Am J Trop Med Hyg. 1988 Nov;39(5):502-10. doi: 10.4269/ajtmh.1988.39.502.

DOI:10.4269/ajtmh.1988.39.502

PMID:3195697

Abstract

A technique was developed for microinjection and transformation of Aedes triseriatus mosquitoes. Mosquito ova can be readily microinjected with approximately 0.9 nl of inoculum if desiccated and inoculated before hardening of the chorion. Ova were injected with molecular constructs that conferred antibiotic (G418) resistance to larvae. Insertion of the antibiotic resistance construct into the mosquito genome was demonstrated by Southern blot analysis.

摘要

开发了一种用于白纹伊蚊微注射和转化的技术。如果在卵壳硬化前对埃及伊蚊卵进行干燥和接种，就可以很容易地用大约0.9纳升的接种物进行微注射。用赋予幼虫抗生素（G418）抗性的分子构建体对卵进行注射。通过Southern印迹分析证明了抗生素抗性构建体已插入蚊子基因组。