Marine College, Shandong University, Weihai, 264209, PR China.
State key Laboratory of Microbial Technology, Shandong University, Qingdao 266237, PR China.
Int J Syst Evol Microbiol. 2020 Mar;70(3):1895-1902. doi: 10.1099/ijsem.0.003990.
A Gram-stain-negative, facultatively aerobic, rod-shaped, motile by gliding and pink-pigmented bacterial strain, designated ELS1360, was isolated from a lake sediment sample collected in Inner Mongolia, PR China. Strain ELS1360 grew optimally at 33 °C, at pH 6.5-7.0 and without NaCl. Strain ELS1360 exhibited 97.3, 97.1 and 96.9 % 16S rRNA gene sequence similarities to HMF3095, JCM 30328 and JCM 30327, respectively, and 90.4-96.9 % to other members of the genus . Results of phylogenetic analysis based on 16S rRNA gene sequences showed that strain ELS1360 belonged to the genus and clustered with JCM 30328 and JCM 30327. The predominant cellular fatty acids were iso-C, summed feature 3 and Cω5. Strain ELS1360 contained MK-7 as the sole menaquinone. The major polar lipids contained phosphatidylethanolamine and two unidentified lipids. The genomic DNA G+C content of strain ELS1360 was 57.1 mol%. Based on the results of our phylogenetic, phenotypic, genotypic and chemotaxonomic analyses, it is concluded that strain ELS1360 represents a novel species within the genus , for which the name sp. nov. is proposed. The type strain is ELS1360 (=KCTC 62449=MCCC 1H00319).
一株革兰氏阴性、兼性需氧、杆状、可滑行运动并呈粉红色的细菌菌株，被命名为 ELS1360，从中国内蒙古的一个湖泊沉积物样本中分离得到。菌株 ELS1360 在 33°C、pH6.5-7.0 和无 NaCl 的条件下生长最佳。菌株 ELS1360 与 HMF3095、JCM 30328 和 JCM 30327 的 16S rRNA 基因序列相似性分别为 97.3%、97.1%和 96.9%，与属内其他成员的相似性为 90.4%-96.9%。基于 16S rRNA 基因序列的系统发育分析结果表明，菌株 ELS1360 属于 属，与 JCM 30328 和 JCM 30327 聚为一簇。主要的细胞脂肪酸为 iso-C、summed feature 3 和 Cω5。菌株 ELS1360 含有 MK-7 作为唯一的menaquinone。主要的极性脂含有磷脂酰乙醇胺和两种未鉴定的脂。菌株 ELS1360 的基因组 DNA G+C 含量为 57.1mol%。基于系统发育、表型、基因型和化学分类学分析的结果，我们认为菌株 ELS1360 代表了 属中的一个新种，建议将其命名为. 模式菌株为 ELS1360（=KCTC 62449=MCCC 1H00319）。
