Arp L H, Hellwig D H
Department of Veterinary Pathology, College of Veterinary Medicine, Iowa State University, Ames 50011.
Avian Dis. 1988 Jul-Sep;32(3):494-500.
Three-week-old turkeys were passively immunized with convalescent serum or treated with tracheal washings from turkeys infected with Bordetella avium. Western blot analysis of the convalescent serum and tracheal washings revealed at least two bands of interaction with outer membrane protein preparations of B. avium. Adherence of B. avium in vivo to tracheal mucosa was determined and compared in treated and untreated turkeys. Passive immunization with convalescent serum reduced adherence of B. avium to the tracheal mucosa in a dose- and time-dependent manner. Adherence was significantly inhibited (P less than 0.01) when turkeys were treated intravenously with 1 ml of undiluted serum either 1 or 6 hours previously. Incubation of the bacterial inoculum with convalescent tracheal washings or application of the washings to tracheal segments before adherence determination in vivo resulted in a significant (P less than 0.01) decrease in adherence. These results indicate that adherence of B. avium to tracheal mucosa is inhibited by substances (antibody) present in both serum and tracheal secretions of convalescent turkeys.
给三周龄的火鸡注射恢复期血清进行被动免疫，或用感染鸟博德特氏菌的火鸡的气管冲洗液进行处理。对恢复期血清和气管冲洗液进行的蛋白质印迹分析显示，与鸟博德特氏菌外膜蛋白制剂至少有两条相互作用带。测定并比较了经处理和未经处理的火鸡体内鸟博德特氏菌对气管黏膜的黏附情况。用恢复期血清进行被动免疫以剂量和时间依赖的方式降低了鸟博德特氏菌对气管黏膜的黏附。如果在1小时或6小时前给火鸡静脉注射1毫升未稀释的血清，黏附会受到显著抑制（P小于0.01）。在体内进行黏附测定之前，将细菌接种物与恢复期气管冲洗液一起孵育或将冲洗液应用于气管段，会导致黏附显著（P小于0.01）降低。这些结果表明，恢复期火鸡的血清和气管分泌物中存在的物质（抗体）可抑制鸟博德特氏菌对气管黏膜的黏附。
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