基于改性二醛纤维素的混合模式色谱固定相的新制备策略。
A new strategy for the preparation of mixed-mode chromatographic stationary phases based on modified dialdehyde cellulose.
机构信息
CAS Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China; University of Chinese Academy of Sciences, Beijing 100049, China.
CAS Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China.
出版信息
J Chromatogr A. 2020 May 10;1618:460885. doi: 10.1016/j.chroma.2020.460885. Epub 2020 Jan 13.
A new strategy for the preparation of mixed-mode chromatographic stationary phases based on modified dialdehyde cellulose was proposed. Two novel mixed-mode chromatographic stationary phases, dicarboxyl cellulose-modified silica (DCC/SiO) and (S)-α-phenylethylamine-bonded DCC/SiO ((S)-α-PEA/DCC/SiO), were prepared by utilizing the easy functionalization characteristics of dialdehyde cellulose. The chromatographic evaluation showed that DCC/SiO column could be used in hydrophilic interaction liquid chromatography (HILIC) and ion exchange chromatography (IEC) modes, (S)-α-PEA/DCC/SiO column could be used in HILIC, IEC and chiral separation modes. The DCC/SiO column and (S)-α-PEA/DCC/SiO column exhibited excellent chromatographic performance by separating strongly polar compounds, phenylamines and chiral compounds in the above separation modes. The preparation method of modified dialdehyde cellulose-based mixed-mode chromatographic stationary phases was simple, and also provided a new idea for the development of the subsequent novel mixed-mode chromatographic stationary phases.
提出了一种基于改性二醛纤维素的混合模式色谱固定相的新制备策略。利用二醛纤维素易功能化的特点，制备了两种新型混合模式色谱固定相，即二羧酸纤维素改性硅胶（DCC/SiO）和（S）-α-苯乙胺键合 DCC/SiO（（S）-α-PEA/DCC/SiO）。色谱评价表明，DCC/SiO 柱可用于亲水作用液相色谱（HILIC）和离子交换色谱（IEC）模式，（S）-α-PEA/DCC/SiO 柱可用于 HILIC、IEC 和手性分离模式。DCC/SiO 柱和（S）-α-PEA/DCC/SiO 柱在上述分离模式下通过分离强极性化合物、苯乙胺和手性化合物，表现出优异的色谱性能。改性二醛纤维素基混合模式色谱固定相的制备方法简单，为后续新型混合模式色谱固定相的开发提供了新思路。
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