Zhou Taicheng, Wu Lili, Zong Zhen, Ma Ning, Li Yingru, Jiang Zhipeng, Wang Qirui, Chen Shuang
Department of Gastroenterological Surgery and Hernia Center, The Sixth Affiliated Hospital of Sun Yat-Sen University, Guangdong Provincial Key Laboratory of Colorectal and Pelvic Floor Disease, Guangzhou, Guangdong 510655, P.R. China.
Department of Ultrasonology, The Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou, Guangdong 510630, P.R. China.
Oncol Lett. 2020 Feb;19(2):1131-1136. doi: 10.3892/ol.2019.11189. Epub 2019 Dec 9.
Long non-coding (lnc)RNA mortal obligate RNA transcript (MORT) is inhibited in numerous types of cancer in humans, indicating its role as a tumor suppressor. The present study demonstrated downregulation of lncRNA MORT in the tumor tissues of patients with colon cancer. The expression of MORT in tumor tissues was linearly associated with its expression levels in plasma. Low MORT expression was associated with low overall survival rate. Moreover, the overexpression of MORT resulted in decreased, whereas treatment with transforming growth factor β1 (TGF-β1) resulted in increased, invasion and migration rates of colon cancer cells. In addition, TGF-β1 treatment attenuated the inhibitory effect of MORT overexpression on the invasion and migration rates of colon cancer cells. The overexpression of MORT inhibited TGF-β1 expression in colon cancer cells, whereas treatment with TGF-β1 failed to affect the expression of the lncRNA. Therefore, it is postulated that MORT inhibits invasion and migration colon cancer cells by inactivating TGF-β1.
长链非编码（lnc）RNA必死性专性RNA转录本（MORT）在人类多种癌症类型中受到抑制，表明其作为肿瘤抑制因子的作用。本研究证明结肠癌患者肿瘤组织中lncRNA MORT表达下调。MORT在肿瘤组织中的表达与其在血浆中的表达水平呈线性相关。MORT低表达与总体生存率低相关。此外，MORT过表达导致结肠癌细胞侵袭和迁移率降低，而用转化生长因子β1（TGF-β1）处理则导致其侵袭和迁移率增加。此外，TGF-β1处理减弱了MORT过表达对结肠癌细胞侵袭和迁移率的抑制作用。MORT过表达抑制结肠癌细胞中TGF-β1表达，而用TGF-β1处理未能影响lncRNA的表达。因此，推测MORT通过使TGF-β1失活来抑制结肠癌细胞的侵袭和迁移。
