Zhang Jimin, Ma Dongzhou, Liu Hui, Wang Jingtao, Fan Jinbiao, Li Xiushan
Department of Neurosurgery, Affiliated Hospital of Hebei University of Engineering Handan 056002, Hebei Province, China.
Department of Neurosurgery, Jizhong Energy Fengfeng Group Hospital Handan 056200, Hebei Province, China.
Int J Clin Exp Pathol. 2017 Nov 1;10(11):10969-10978. eCollection 2017.
MicroRNAs (miRNAs) are a class of small non-coding RNA molecules, about 21-25 nucleotides in length. Accumulating evidence demonstrated that dysregulation or dysfunction of miRNAs are involved in various diseases, including cancer. MiR-143, recently has been reported to function as an important tumor suppressor in prostate cancer, pancreatic ductal adenocarcinoma and other kinds of cancers, but rarely systematically studied in pituitary tumor. In the present study, we firstly found that miR-143 was significantly down-regulated in pituitary tumor tissues and cell lines (GH3 and MMQ). Then, subsequent studies revealed that miR-143 inhibits cell proliferation and promotes apoptosis in both GH3 and MMQ cells. In addition, K-Ras, one of the most important oncogenes involved in many kinds of cancers, was found to be suppressed by miR-143 in pituitary tumor. Furthermore, overexpression of K-Ras greatly reversed the suppressive effect of miR-143 on pituitary tumor cells. In summary, our study demonstrated that miR-143 functions as a tumor suppressor and directly targets K-Ras in human pituitary tumor.
微小RNA（miRNA）是一类小的非编码RNA分子，长度约为21 - 25个核苷酸。越来越多的证据表明，miRNA的失调或功能障碍与包括癌症在内的各种疾病有关。最近有报道称，miR - 143在前列腺癌、胰腺导管腺癌和其他类型的癌症中作为一种重要的肿瘤抑制因子发挥作用，但在垂体瘤中很少进行系统研究。在本研究中，我们首先发现miR - 143在垂体瘤组织和细胞系（GH3和MMQ）中显著下调。随后的研究表明，miR - 143在GH3和MMQ细胞中均抑制细胞增殖并促进细胞凋亡。此外，发现K - Ras是参与多种癌症的最重要的癌基因之一，在垂体瘤中被miR - 143抑制。此外，K - Ras的过表达大大逆转了miR - 143对垂体瘤细胞的抑制作用。总之，我们的研究表明，miR - 143在人垂体瘤中作为肿瘤抑制因子发挥作用，并直接靶向K - Ras。
