Zhang Lin, Zhang Feng, He Daikun, Xu Daojian, Zhong Zhiyue, Shen Jie
Center of Emergency & Intensive Care Unit, Jinshan Hospital, Fudan University Shanghai 201508, P. R. China.
Medical Center of Chemical Injury, Jinshan Hospital, Fudan University Shanghai 201508, P. R. China.
Int J Clin Exp Pathol. 2017 Nov 1;10(11):11281-11287. eCollection 2017.
The aim of this study was to assess the therapeutic effect of melatonin in phosgene induced acute lung injury (ALI) and to explore the related mechanisms. A rat model of phosgene induced ALI was established and the severity of the ALI was evaluated by wet/dry (W:D) ratio of lung weight, bronchoalveolar lavage (BAL) fluid cell counts and inflammatory cytokines. The rats were administrated with elatonin (MT), ulinastatin (UTI), p38 inhibitor and NF-κB inhibitor pyrrolidine dithiocarbamate (PDTC) alone or in combination. We found that MT in combination with UTI significantly improved the severity of ALI and the activation of Wnt/β-catenin signaling was involved in beneficial effect of MT. MT may be used as a therapeutic adjuvant for phosgene induced ALI.
本研究旨在评估褪黑素对光气诱导的急性肺损伤（ALI）的治疗效果，并探讨其相关机制。建立了光气诱导的ALI大鼠模型，并通过肺重量湿/干（W:D）比值、支气管肺泡灌洗（BAL）液细胞计数和炎性细胞因子评估ALI的严重程度。大鼠单独或联合给予褪黑素（MT）、乌司他丁（UTI）、p38抑制剂和NF-κB抑制剂吡咯烷二硫代氨基甲酸盐（PDTC）。我们发现，MT与UTI联合使用可显著改善ALI的严重程度，且Wnt/β-连环蛋白信号通路的激活参与了MT的有益作用。MT可作为光气诱导的ALI的治疗辅助药物。
