Martina P F, Martinez M, Centeno C K, VON Specht M, Ferreras J
Facultad de Ciencias Exactas, Químicas y Naturales, Universidad Nacional de Misiones, Argentina.
Instituto de Biología Subtropical (IBS), CONICET-UNaM, CCT-Nordeste, Argentina.
J Prev Med Hyg. 2019 Dec 20;60(4):E293-E299. doi: 10.15167/2421-4248/jpmh2019.60.4.1283. eCollection 2019 Dec.
It is recognized that mobile phones may play a role in microorganism transmission and that hand hygiene, is considered the most important action for preventing infections and the spread of pathogens. The objective of this study was to determine presence and circulation bacteria on hands and mobile phones capable of causing infections in people and also determine if disinfection with gel-alcohol is useful to reduce the bacterial colonization.
The bacterial evaluation included 596 hands of participants and 256 mobile phones. Isolated colonies were identified by biochemical test and confirmed by gene 16S rRNA sequencing. Antimicrobial susceptibility was performed using the automated instrument Vitek®2-Compact and disk-diffusionmethod.
In total, 92.9% of mobile phones and 98.3% of participants in study demonstrated evidence of bacterial contamination with different types of bacteria. Surprisingly, we observed that 18.6% plaques inoculated with disinfected fingers showed bacterial growth. In general, Gram negative isolates showed resistance to a higher number of antibiotics tested than Gram positive isolates.
Our results could help to raise awareness in our society about the importance of hand hygiene, as well as frequently used devices, reducing bacterial contamination and limiting the possibility of transmission of resistant multi-drug bacteria.
人们认识到手机可能在微生物传播中起作用，而手部卫生被认为是预防感染和病原体传播的最重要措施。本研究的目的是确定手上和手机上能够导致人类感染的细菌的存在和传播情况，并确定使用酒精凝胶进行消毒是否有助于减少细菌定植。
细菌评估包括596名参与者的手部和256部手机。通过生化试验鉴定分离出的菌落，并通过16S rRNA基因测序进行确认。使用自动化仪器Vitek®2-Compact和纸片扩散法进行药敏试验。
总体而言，92.9%的手机和98.3%的研究参与者显示出不同类型细菌污染的证据。令人惊讶的是，我们观察到接种了消毒手指的菌斑中有18.6%显示出细菌生长。一般来说，革兰氏阴性菌分离株比革兰氏阳性菌分离株对更多测试抗生素耐药。
我们的结果有助于提高社会对手部卫生以及常用设备重要性的认识，减少细菌污染并限制多重耐药菌传播的可能性。
