在厩螫蝇吸食持续感染牛的血液后检测牛病毒性腹泻病毒。
Detection of bovine viral diarrhea virus in stable flies following consumption of blood from persistently infected cattle.
作者信息
Carlson Jaden M, Vander Ley Brian L, Lee Sang I, Grotelueschen Dale M, Walz Paul H, Workman Aspen M, Heaton Michael P, Boxler David J
机构信息
Department of Veterinary Medicine and Biomedical Sciences, Great Plains Veterinary Educational Center, University of Nebraska-Lincoln, Clay Center, NE (Carlson, Vander Ley, Grotelueschen).
Department of Food Science and Technology, Oregon State University, Corvallis, OR (Lee).
出版信息
J Vet Diagn Invest. 2020 Jan;32(1):108-111. doi: 10.1177/1040638719898688. Epub 2020 Jan 22.
Control of bovine viral diarrhea virus (BVDV) relies on resource-intensive sampling to detect and remove persistently infected (PI) cattle. Herd-level surveillance tools would be useful for herds with unknown BVDV status and for monitoring herds with BVDV-free status. Our objective was to determine the feasibility of using stable flies as a sampling tool to detect BVDV at the herd level. Stable flies () were fed citrated blood from either BVDV-PI or BVDV-free cattle to establish pools of 100 flies with various proportions of BVDV-fed flies (0%, 1%, 10%, 20%, 40%, or 100% in each pool). BVDV-fed flies in these pools were harvested either 1, 2, or 3 d after consuming BVDV-PI blood to determine the impact of time after feeding. Two replicates of a 3-d by 6-dilution level matrix were produced. BVDV RNA was consistently detected on day 1 when ≥10% of the flies in the pool consumed PI blood. On days 2 and 3, positive BVDV RNA detection was variable and became less consistent. Our results demonstrate that BVDV RNA can be detected in stable flies after feeding on blood from PI cattle. Successful use of stable flies as a surveillance tool will require validation under field conditions.
牛病毒性腹泻病毒（BVDV）的防控依赖于资源密集型采样，以检测和清除持续感染（PI）的牛。群体水平监测工具对于BVDV状况未知的牛群以及监测无BVDV牛群将很有用。我们的目标是确定使用厩螫蝇作为采样工具在群体水平检测BVDV的可行性。给厩螫蝇（）喂食来自BVDV-PI牛或无BVDV牛的枸橼酸化血液，以建立含有不同比例喂食BVDV血液苍蝇的100只苍蝇池（每个池中0%、1%、10%、20%、40%或100%）。这些池中喂食BVDV的苍蝇在消耗BVDV-PI血液后1、2或3天收获，以确定喂食后时间的影响。制作了一个3天×6稀释水平矩阵的两个重复样本。当池中≥10%的苍蝇消耗PI血液时，在第1天始终能检测到BVDV RNA。在第2天和第3天，BVDV RNA阳性检测结果不稳定且一致性降低。我们的结果表明，厩螫蝇在吸食PI牛的血液后可以检测到BVDV RNA。要成功将厩螫蝇用作监测工具，需要在田间条件下进行验证。
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