Department of Clinical Sciences, College of Veterinary Medicine, Auburn University, Auburn, AL 36849, USA.
Vet Res Commun. 2011 Feb;35(2):123-9. doi: 10.1007/s11259-010-9453-7. Epub 2010 Dec 7.
Identifying reservoirs and transmission routes for bovine viral diarrhea virus (BVDV) are important in developing biosecurity programs. The aim of this study was to evaluate BVDV transmission by the hematophagous horn fly (Haematobia irritans). Flies collected from four persistently infected cattle were placed in fly cages attached to principal (n = 4) and control (n = 4) BVDV-naïve calves housed individually in isolation rooms. Flies were able to feed on principal calves, but a barrier prevented fly feeding from control calves. Flies were tested for BVDV by RT-PCR and virus isolation at time of collection from PI cattle and after 48 h of exposure on BVDV-naïve calves. Blood samples were collected from calves and tested for BVDV infection. Virus was isolated from fly homogenates at collection from PI animals and at removal from control and principal calves. All calves remained negative for BVDV by virus isolation and serology throughout the study. Bovine viral diarrhea virus may be detected in horn flies collected from PI cattle, but horn flies do not appear to be an important vector for BVDV transmission.
鉴定牛病毒性腹泻病毒（BVDV）的储存宿主和传播途径对于制定生物安全计划非常重要。本研究旨在评估吸血性牛角蝇（Haematobia irritans）传播 BVDV 的能力。从 4 头持续感染的牛中收集的苍蝇被放置在附有主（n=4）和对照（n=4）BVDV 初免小牛的蝇笼中，这些小牛单独隔离在隔离室内。苍蝇能够以主小牛为食，但有一个屏障阻止苍蝇以对照小牛为食。从 PI 牛收集时以及暴露在 BVDV 初免小牛上 48 小时后，通过 RT-PCR 和病毒分离试验检测苍蝇中的 BVDV。从小牛采集血液样本并检测 BVDV 感染情况。从 PI 动物采集的蝇匀浆以及从对照和主小牛上移除时，均可分离到病毒。在整个研究过程中，所有小牛在病毒分离和血清学检测方面均持续呈 BVDV 阴性。从 PI 牛中采集的牛角蝇中可能检测到牛病毒性腹泻病毒，但牛角蝇似乎不是 BVDV 传播的重要媒介。
