Bassett S G, Pepe G J
Department of Physiology, Eastern Virginia Medical School, Norfolk 23501.
Biol Reprod. 1988 Oct;39(3):526-31. doi: 10.1095/biolreprod39.3.526.
The metabolism of testosterone (T) was examined during the second half of pregnancy in the rat to determine whether utilization of T for estradiol (E2) synthesis occurs via conversion of T to androstenedione (A). On Days 11, 16, and 21 of gestation (term = Day 23), rats (n = 7-9/group) were anesthetized and a constant infusion of [3H]T was initiated. At 60 min, blood was obtained from a jugular vein and the ovaries (Days 11, 16, and 21), and placentae and uterine tissue (Day 16 only) were removed. In a second study performed in rats on Day 16 of gestation (n = 8-10/group), the ovaries and/or gravid uterus were removed 15 min after initiation of [3H]T infusion, and blood was taken from a jugular vein 60 min later. Radiolabeled T and A were purified from serum and tissues by paper chromatography. In a third group of rats (n = 6), jugular vein samples were obtained sequentially on Days 11, 16 and 21 of gestation and serum concentrations of T were measured by radioimmunoassay. The metabolic clearance rate of T was constant during the study period (overall mean = 31 1/day). In contrast, the serum concentration of T (pg/ml) on Day 16 of gestation (863 +/- 108) exceeded (p less than 0.02) that on Day 11 (445 +/- 74); the latter was similar to that measured on Day 21 (592 +/- 109). Thus, the estimated production rate of T was greatest on Day 16 of gestation.(ABSTRACT TRUNCATED AT 250 WORDS)
为了确定睾酮（T）用于合成雌二醇（E2）是否通过T转化为雄烯二酮（A）来实现，对大鼠妊娠后半期的T代谢情况进行了研究。在妊娠第11、16和21天（足月为第23天），将大鼠（每组n = 7 - 9只）麻醉，并开始持续输注[3H]T。60分钟时，从颈静脉取血，并摘除卵巢（第11、16和21天）以及胎盘和子宫组织（仅第16天）。在另一项对妊娠第16天的大鼠进行的研究中（每组n = 8 - 10只），在开始输注[3H]T 15分钟后摘除卵巢和/或妊娠子宫，60分钟后从颈静脉取血。通过纸色谱法从血清和组织中纯化放射性标记的T和A。在第三组大鼠（n = 6只）中，在妊娠第11、16和21天依次采集颈静脉样本，并通过放射免疫测定法测量血清T浓度。在研究期间，T的代谢清除率保持恒定（总体平均值 = 31 l/天）。相比之下，妊娠第16天的血清T浓度（pg/ml）（863 ± 108）超过（p < 0.02）第11天（445 ± 74）；后者与第21天测量的值（592 ± 109）相似。因此，妊娠第16天T的估计产生率最高。（摘要截短至250字）
