Laboratory of Microbiology and Immunology, Ciencia de los Alimentos, Centro de Investigación en Alimentación y Desarrollo A. C. (CIAD). Carretera Gustavo Astiazarán Rosas 46, Col. la Victoria, Hermosillo 83304, Sonora, Mexico.
Laboratory of Immunology, Centro de Investigación en Alimentación y Desarrollo A. C. (CIAD), México, Carretera Gustavo Astiazarán Rosas 46, Col. la Victoria, Hermosillo 83304, Sonora, Mexico.
Int J Mol Sci. 2020 Jan 21;21(3):687. doi: 10.3390/ijms21030687.
MicroRNAs (miRNAs) mediate the regulation of gene expression. Several reports indicate that probiotics induce miRNA-mediated immunomodulation at different levels, such as cytokine production and the up-regulation of several markers related to antigen presentation in antigen-presenting cells. The objective of this work was to identify target genes of miRNAs that are involved in the processing and presentation of antigens in monocyte-derived dendritic cells (moDCs) stimulated with the probiotic ssp BB12 (BB12). First, an in silico prediction analysis for a putative miRNA binding site within a given mRNA target was performed using RNAHybrid software with mature sequences of differentially expressed miRNAs retrieved from a Genbank data set that included BB12-stimulated and unstimulated porcine monocytes. From them, 23 genes resulted in targets of 19 miRNAs, highlighting miR-30b-3p, miR-671-5p, and miR-9858-5p, whose targets were costimulatory molecules, and were overexpressed ( < 0.05) in BB12-stimulated moDCs. The analysis of moDCs showed that the percentage of cells expressing SLA-DRCD80 decreased significantly ( = 0.0081) in BB12-stimulated moDCs; interleukin (IL)-10 production was unchanged at 6 h but increased after 24 h of culture in the presence of BB12 ( < 0.001). In summary, our results suggest that SLA-DR and CD80 can be down-regulated by miRNAs miR-30b-3p, miR-671-5p, and miR-9858-5p, while miR-671-5p targets IL-10.
微小 RNA（miRNA）介导基因表达的调控。有几项报告表明，益生菌在不同水平诱导 miRNA 介导的免疫调节，如细胞因子的产生和抗原呈递细胞中与抗原呈递相关的几种标志物的上调。本工作的目的是鉴定参与单核细胞衍生树突状细胞（moDC）中抗原加工和呈递的 miRNA 的靶基因，该细胞用益生菌 ssp BB12（BB12）刺激。首先，使用 RNAHybrid 软件对给定 mRNA 靶标中 miRNA 结合位点的可能进行了计算机预测分析，使用的成熟序列是从包括 BB12 刺激和未刺激猪单核细胞的 Genbank 数据集检索到的差异表达 miRNA。其中，23 个基因成为 19 个 miRNA 的靶标，突出了 miR-30b-3p、miR-671-5p 和 miR-9858-5p，它们的靶标是共刺激分子，在 BB12 刺激的 moDC 中过度表达（<0.05）。对 moDC 的分析表明，表达 SLA-DRCD80 的细胞百分比在 BB12 刺激的 moDC 中显著降低（=0.0081）；在 BB12 存在的情况下培养 6 小时后，白细胞介素（IL）-10 的产生没有变化，但在 24 小时后增加（<0.001）。总之，我们的结果表明，miR-30b-3p、miR-671-5p 和 miR-9858-5p 可以下调 SLA-DR 和 CD80，而 miR-671-5p 的靶标是 IL-10。
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