Arenas-Padilla M, Duarte-Gutiérrez J L, Mata-Haro V
Department of Food Science, Microbiology and Immunology, Centro de Investigación en Alimentación y Desarrollo, A. C., Hermosillo, México.
J Appl Microbiol. 2018 Dec 1;125(6):1881-9. doi: 10.1111/jam.14069. Epub 2018 Aug 14.
To investigate the role of Toll-like receptor 2 (TLR2) in interleukin-10 (IL-10) production induced by Bifidobacterium animalis ssp. lactis Bb12 (Bb12) in swine immune cells.
Blood-monocytes and cells from mesenteric lymph nodes were obtained from pigs and cultured with live Bb12 for 4 and 12 h. Transcript levels of IL-10 and TLR2 were analysed. Furthermore, TLR2 was blocked to determine its participation in IL-10 production. TLR2 blockade was achieved with neutralizing antibodies, followed by stimulation with Bb12. Bifidobacteria induced IL-10 production in both swine monocytes and mesenteric cells. Monocytes with TLR2 blockade had a decrease in IL-10 transcripts, while mesenteric cells did not. Bacterial cell wall components were responsible for Bb12-induced IL-10 production since no IL-10 was detected in the culture supernatant.
We demonstrated that IL-10 production is largely mediated through the recognition of Bb12 structures by TLR2, as bacterial metabolites in the culture supernatant failed to induce IL-10 expression.
The present study provides evidence for the potential use of Bb12 in the swine industry; these bacteria can also be used as additional method to treat intestinal inflammation and enhance intestinal health in pigs.
研究Toll样受体2（TLR2）在动物双歧杆菌乳酸亚种Bb12（Bb12）诱导猪免疫细胞产生白细胞介素-10（IL-10）过程中的作用。
从猪身上获取血液单核细胞和肠系膜淋巴结细胞，并用活的Bb12培养4小时和12小时。分析IL-10和TLR2的转录水平。此外，阻断TLR2以确定其在IL-10产生中的参与情况。用中和抗体实现TLR2阻断，然后用Bb12刺激。双歧杆菌在猪单核细胞和肠系膜细胞中均诱导IL-10产生。TLR2被阻断的单核细胞中IL-10转录本减少，而肠系膜细胞中则没有。细菌细胞壁成分是Bb12诱导IL-10产生的原因，因为在培养上清液中未检测到IL-10。
我们证明，由于培养上清液中的细菌代谢产物未能诱导IL-10表达，IL-10的产生很大程度上是通过TLR2识别Bb12结构介导的。
本研究为Bb12在养猪业中的潜在应用提供了证据；这些细菌还可作为治疗猪肠道炎症和增强肠道健康的额外方法。
