里氏木霉的α,α-海藻糖酶
alpha,alpha-Trehalase of Trichoderma reesei.
作者信息
Vijayakumar P, Ross W, Reese E T
出版信息
Can J Microbiol. 1978 Oct;24(10):1280-3. doi: 10.1139/m78-206.
DOI:10.1139/m78-206
PMID:31976
Abstract
A simple adsorption and elution of the trehalase of Trichoderma reesei on bentonite increased the specific activity 70-80 times, with a recovery of 90%. This alpha,alpha-trehalase has an optimum pH of 4.4, a pl of 5.7, a Km of 3.1 X 10(-3) M, and a specific activity of 50 mumol/mg. min-1.
摘要
里氏木霉海藻糖酶在膨润土上进行简单的吸附和洗脱后，比活性提高了70 - 80倍，回收率为90%。这种α,α-海藻糖酶的最适pH为4.4，等电点为5.7，米氏常数为3.1×10⁻³ M，比活性为50 μmol/(mg·min)。
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