Bette-Bobillo P, Bienvenue A
Laboratoire de Biologie Physico-Chimique, Unité Associée 530 du Centre National de la Recherche Scientifique, Université des Sciences et Techniques du Languedoc, Montpellier, France.
Eur J Biochem. 1988 Nov 15;177(3):643-7. doi: 10.1111/j.1432-1033.1988.tb14418.x.
Using a radioimmunoassay for rabbit-serum albumin, platelet-activating-factor-induced serum-albumin release by platelets was monitored under non-aggregating conditions. The four main results from this study are as follows. The EC50 of the release was of the same order of magnitude as the aggregation EC50 in the presence of calcium. The release took place within 2 min and was inhibited by BN 52021, which is a very specific inhibitor of the platelet-activating-factor-aggregating effect. Serum-albumin release was much greater than serotonin release.
采用兔血清白蛋白放射免疫分析法，在非聚集条件下监测血小板活化因子诱导的血小板血清白蛋白释放。本研究的四个主要结果如下。释放的半数有效浓度（EC50）与存在钙时聚集的EC50处于同一数量级。释放发生在2分钟内，并被BN 52021抑制，BN 52021是血小板活化因子聚集效应的一种非常特异性的抑制剂。血清白蛋白释放比血清素释放大得多。
