Laboratory of Developmental Nutrition, Department of Animal Sciences, North Carolina State University, Raleigh, NC 27695, USA.
Int J Mol Sci. 2020 Jan 22;21(3):726. doi: 10.3390/ijms21030726.
Maintaining an active fatty acid metabolism is important for renal growth, development, and health. We evaluated the effects of anaplerotic and ketogenic energy sources on fatty acid oxidation during stimulation with clofibrate, a pharmacologic peroxisome proliferator-activated receptor α (PPARα) agonist. Suckling newborn pigs ( = 72) were assigned into 8 dietary treatments following a 2 × 4 factorial design: ± clofibrate (0.35%) and diets containing 5% of either (1) glycerol-succinate (GlySuc), (2) tri-valerate (TriC5), (3) tri-hexanoate (TriC6), or (4) tri-2-methylpentanoate (Tri2MPA). Pigs were housed individually and fed the iso-caloric milk replacer diets for 5 d. Renal fatty acid oxidation was measured in vitro in fresh tissue homogenates using [1-C]-labeled palmitic acid. The oxidation was 30% greater in pig received clofibrate and 25% greater ( < 0.05) in pigs fed the TriC6 diet compared to those fed diets with GlySuc, TriC5, and Tri2MPA. Addition of carnitine also stimulated the oxidation by twofold ( < 0.05). The effects of TriC6 and carnitine on palmitic acid oxidation were not altered by clofibrate stimulation. However, renal fatty acid composition was altered by clofibrate and Tri2MPA. In conclusion, modification of anaplerosis or ketogenesis via dietary substrates had no influence on in vitro renal palmitic acid oxidation induced by PPARα activation.
维持活跃的脂肪酸代谢对于肾脏的生长、发育和健康非常重要。我们评估了补料和生酮能源对氯贝特刺激期间脂肪酸氧化的影响，氯贝特是一种药理学过氧化物酶体增殖物激活受体 α（PPARα）激动剂。新生仔猪（=72）根据 2×4 析因设计分为 8 种饮食处理：氯贝特（0.35%）和含有 5%甘油琥珀酸酯（GlySuc）、三丁酸酯（TriC5）、三己酸酯（TriC6）或三-2-甲基戊酸酯（Tri2MPA）的饮食。猪单独饲养并喂食等热量的代乳料 5 天。在新鲜组织匀浆中通过 [1-C]-标记棕榈酸测量肾脏脂肪酸氧化。与喂食 GlySuc、TriC5 和 Tri2MPA 的猪相比，接受氯贝特和 TriC6 饮食的猪的氧化作用分别增加了 30%（<0.05）和 25%（<0.05）。添加肉碱也使氧化作用增加了两倍（<0.05）。TriC6 和肉碱对棕榈酸氧化的影响不受氯贝特刺激的改变。然而，氯贝特和 Tri2MPA 改变了肾脏脂肪酸的组成。总之，通过饮食底物对氨生成或生酮作用的修饰对 PPARα 激活诱导的肾脏棕榈酸氧化的体外无影响。
