性腺类固醇对火鸡垂体前叶细胞基础状态及血管活性肠肽刺激的催乳素释放的调节作用。

Gonadal steroid modulation of basal and vasoactive intestinal polypeptide-stimulated prolactin release by turkey anterior pituitary cells.

作者信息

Knapp T R, Fehrer S C, Silsby J L, Porter T E, Behnke E J, el Halawani M E

机构信息

Department of Animal Science, University of Minnesota, St. Paul 55108.

出版信息

Gen Comp Endocrinol. 1988 Nov;72(2):226-36. doi: 10.1016/0016-6480(88)90205-5.

DOI:10.1016/0016-6480(88)90205-5

PMID:3197943

Abstract

Porcine vasoactive intestinal polypeptide (VIP; 10(-9) to 10(-7) M) was a potent stimulator of prolactin (PRL) release by anterior pituitary cells from immature and laying turkey hens. Basal and VIP-induced PRL release of cells from laying hens were diminished (P less than 0.05) when the cells were cultured for 48 hr in the presence of charcoal-stripped laying hen serum, but not when the cells were cultured in the presence of whole laying hen serum. This change in VIP-induced PRL release was not evident when cells were derived from immature hens. Basal PRL release by cells from laying hens was not altered by the presence of estradiol (E2; 10(-12) to 10(-5) M), although such release was generally enhanced in cultures of cells from immature hens containing E2. The presence of E2 enhanced (P less than 0.05) the magnitude of the VIP-induced PRL release by cultures of cells from laying hens and diminished (P less than 0.05) the magnitude of this release in cultures of cells from immature hens. Cells from immature and laying hens exposed to progesterone (P4; 10(-5) M) for 96 hr exhibited enhanced basal PRL release, though lower P4 concentrations had no effect. Utilizing cells from laying hens, P4 exposure for 24 hr resulted in diminished (P less than 0.05) VIP-induced PRL release, while P4 exposure for 96 hr resulted in markedly enhanced (P less than 0.05) VIP-induced PRL release. Basal PRL release was generally not altered by the presence of testosterone (T). The VIP-induced PRL release by cells derived from immature and laying hens was diminished (P less than 0.05) by the presence of T. Prolactin release in the turkey is likely modulated by gonadal steroids acting directly on the cells of the anterior pituitary.

摘要

猪血管活性肠肽（VIP；10⁻⁹至10⁻⁷M）是未成熟和产蛋母火鸡垂体前叶细胞催乳素（PRL）释放的强效刺激剂。当细胞在经活性炭处理的产蛋母鸡血清存在下培养48小时时，产蛋母鸡细胞的基础PRL释放和VIP诱导的PRL释放均减少（P<0.05），但当细胞在全产蛋母鸡血清存在下培养时则不然。当细胞来自未成熟母鸡时，VIP诱导的PRL释放的这种变化不明显。产蛋母鸡细胞的基础PRL释放不受雌二醇（E2；10⁻¹²至10⁻⁵M）存在的影响，尽管在含有E2的未成熟母鸡细胞培养物中这种释放通常会增强。E2的存在增强了（P<0.05）产蛋母鸡细胞培养物中VIP诱导的PRL释放幅度，并降低了（P<0.05）未成熟母鸡细胞培养物中这种释放的幅度。暴露于孕酮（P4；10⁻⁵M）96小时的未成熟和产蛋母鸡细胞表现出基础PRL释放增强，尽管较低的P4浓度没有影响。利用产蛋母鸡的细胞，暴露于P4 24小时导致VIP诱导的PRL释放减少（P<0.05），而暴露于P4 96小时导致VIP诱导的PRL释放显著增强（P<0.05）。基础PRL释放通常不受睾酮（T）存在的影响。T的存在使未成熟和产蛋母鸡来源的细胞的VIP诱导的PRL释放减少（P<0.05）。火鸡中的催乳素释放可能受到直接作用于垂体前叶细胞的性腺类固醇的调节。