Berger T, Horton M B
Department of Animal Science, University of California, Davis 95616.
Gamete Res. 1988 Jan;19(1):101-11. doi: 10.1002/mrd.1120190110.
The ability to penetrate zona-free hamster ova may be a very useful test of fresh and frozen boar sperm fertility. These studies were designed to optimize assay conditions prior to evaluation of the accuracy of the bioassay in predicting boar sperm fertility. The ability to penetrate zona-free hamster ova was greater in sperm washed on a Percoll gradient than in sperm washed by dilution and centrifugation. Penetrating ability was greater in sperm from the sperm-rich fraction than from the whole ejaculate but did not differ among different aliquots of the sperm-rich fraction and did not decrease when the prewashing interval was increased from 15 to 85 min. Frequency of collection of ejaculates (1, 3, or 5 times per week) did not affect the penetrating ability of the sperm. Penetration rate was greater when sperm were coincubated with zona-free hamster ova at 39 degrees C compared to 37 degrees C. Sperm from an infertile boar had reduced penetrating ability compared to sperm from fertile boars (11% vs 93%, P less than .001). These studies suggest that the zona-free hamster ova bioassay may be a useful assessment of fresh boar sperm fertility.
穿透去透明带仓鼠卵的能力可能是检测新鲜和冷冻公猪精子受精能力的一项非常有用的试验。这些研究旨在优化试验条件，然后再评估该生物测定法在预测公猪精子受精能力方面的准确性。经Percoll梯度洗涤的精子穿透去透明带仓鼠卵的能力，比经稀释和离心洗涤的精子更强。富含精子部分的精子穿透能力，比整个射精样本的精子更强，但富含精子部分的不同等分样本之间没有差异，并且当预洗涤间隔从15分钟增加到85分钟时，穿透能力也没有下降。射精样本的采集频率（每周1次、3次或5次）不影响精子的穿透能力。与37℃相比，精子在39℃下与去透明带仓鼠卵共同孵育时，穿透率更高。与可育公猪的精子相比，不育公猪的精子穿透能力降低（11% 对93%，P < 0.001）。这些研究表明，去透明带仓鼠卵生物测定法可能是评估新鲜公猪精子受精能力的一种有用方法。
