Wraith M J, Watson W P, Eadsforth C V, van Sittert N J, Törnqvist M, Wright A S
Shell Research Ltd., Sittingbourne Research Centre, Kent, UK.
IARC Sci Publ. 1988(89):271-4.
A novel immunochemical approach has been developed to monitor human exposures to ethylene oxide (EO). The method exploits the interaction of EO with the amino function of the N-terminal valine residue of the alpha-chain of human haemoglobin (Hb). Antibodies were raised against the adducted valine in the form of the N-terminal tryptic heptapeptide and have been used to develop a sensitive radioimmunoassay (RIA) for the adducted heptapeptide. This method has been fully validated against a gas chromatography-mass spectrometry (GC-MS) method and has been applied to the monitoring of EO exposure in a group of sterilization workers.
已开发出一种新型免疫化学方法来监测人体对环氧乙烷（EO）的暴露情况。该方法利用了EO与人类血红蛋白（Hb）α链N端缬氨酸残基的氨基功能之间的相互作用。针对以N端胰蛋白酶七肽形式存在的加合缬氨酸产生了抗体，并已用于开发针对加合七肽的灵敏放射免疫分析（RIA）。该方法已通过气相色谱 - 质谱（GC-MS）方法进行了全面验证，并已应用于一组灭菌工人的EO暴露监测。
