Saha M, Minnetian O, Fisher D, Rogers E, Annan R, Kresbach G, Vouros P, Giese R
Department of Medicinal Chemistry, College of Pharmacy and Allied Health Professions, Northeastern University, Boston, MA.
IARC Sci Publ. 1988(89):356-60.
The DNA adduct O4-ethylthymine can be alkylated under mild conditions with pentafluorobenzyl bromide. The product has good gas chromatographic characteristics and also forms a structurally characteristic anion in high yield when subjected to electron capture mass spectrometry. Related adducts for other DNA bases behave similarly. These properties stimulated us to develop a general analytical method based on the derivatization reaction. Important for this method is an oxidation-elimination reaction that mildly releases a base from a nucleoside. Thus, a general analytical method based on pentafluorobenzylation is now available for determining many alkyl and related DNA adducts.
DNA加合物O4 - 乙基胸腺嘧啶在温和条件下可被五氟苄基溴烷基化。该产物具有良好的气相色谱特性，并且在进行电子捕获质谱分析时能高产率地形成结构特征性阴离子。其他DNA碱基的相关加合物表现类似。这些特性促使我们基于衍生化反应开发一种通用分析方法。该方法的关键是一种氧化消除反应，可温和地从核苷中释放出碱基。因此，现在有一种基于五氟苄基化的通用分析方法可用于测定多种烷基及相关DNA加合物。
