唑来膦酸抑制脂肪来源干细胞向成骨细胞分化潜能在修复颌骨坏死中的作用。
Zoledronate inhibits the differentiation potential of adipose-derived stem cells into osteoblasts in repairing jaw necrosis.
机构信息
Department of Oral & Maxillofacial-Head Neck Oncology, Ninth People's Hospital, College of Stomatology, Shanghai JiaoTong University, School of Medicine, Shanghai, 200011, China.
Department of Oral & Maxillofacial-Head Neck Oncology, Ninth People's Hospital, College of Stomatology, Shanghai JiaoTong University, School of Medicine, Shanghai, 200011, China.
出版信息
Mol Cell Probes. 2020 Jun;51:101525. doi: 10.1016/j.mcp.2020.101525. Epub 2020 Jan 23.
OBJECTIVE
To explore the inhibitory effects of zoledronate (ZOL) on adipose-derived stem cells (ADSCs) into osteoblasts for repairing jaw necrosis.
METHODS
ADSCs were induced to differentiate into osteoblasts. The differentiation characteristics of osteoblasts was observed under inverted microscope by alizarin red staining. The transwell assay was performed to evaluate the migration of ADSCs co-cultured with osteoblasts and divided into ZOL group treated with ZOL and N-ZOL group without ZOL treatment. The differentiation and proliferation characteristics of ADSCs differentiated osteoblasts were observed respectively. The expression of CTSK (Cathepsin K) and FGFR3 (Fibroblast growth factor receptor 3) in osteoblasts were analyzed by immunofluorescence and western blot.
RESULTS
The differentiation degree and proliferation of ADSCs to osteoblasts in N-ZOL group were both higher than those in ZOL group. The migratory cell number in ADSCs differentiation in ZOL group was higher than that of N-ZOL group. The protein expression of CTSK and FGFR3 in ADSCs differentiated to osteoblasts in ZOL group was higher than that in N-ZOL group.
CONCLUSION
The differentiation of ADSCs into osteoblasts is significantly inhibited by ZOL. Due to this reason, it may be difficult to achieve good results by ZOL induced ADSCs into osteoblasts in repairing jaw necrosis.
目的
探索唑来膦酸（zoledronate，ZOL）对脂肪间充质干细胞（adipose-derived stem cells，ADSCs）向成骨细胞分化的抑制作用，用于修复颌骨坏死。
方法
诱导 ADSCs 向成骨细胞分化，倒置显微镜下茜素红染色观察成骨细胞分化特征。Transwell 小室实验检测共培养条件下成骨细胞对 ADSCs 迁移的影响，分为 ZOL 处理组（加入 ZOL）和未处理组（N-ZOL 组）。观察 ADSCs 向成骨细胞分化的增殖和分化特征。免疫荧光和 Western blot 检测成骨细胞中组织蛋白酶 K（Cathepsin K）和成纤维细胞生长因子受体 3（Fibroblast growth factor receptor 3，FGFR3）的表达。
结果
N-ZOL 组 ADSCs 向成骨细胞的分化程度和增殖能力均高于 ZOL 组，ZOL 组 ADSCs 向成骨细胞的迁移细胞数高于 N-ZOL 组，ZOL 组 ADSCs 向成骨细胞分化的 CTSK 和 FGFR3 蛋白表达高于 N-ZOL 组。
结论
ZOL 显著抑制 ADSCs 向成骨细胞分化，可能导致 ZOL 诱导 ADSCs 向成骨细胞修复颌骨坏死效果不佳。
Zotero 插件